Material Transfer Agreements

Please note that after 10 years of providing these cell lines and reagents to several thousand laboratories worldwide at the Nolan Lab expense, we have changed our procedures for providing these cells and vectors.

Academic and non-profit laboratories are not required to contact the Nolan laboratory for obtaining these materials.

Note: After July 15, 2005, any requests using the older MTA forms (which are no longer available) will be returned and you will be directed to the websites below.

Companies and industrial institutes should direct inquiries by e-mail or letter directly to:

Howard Guss

Academic and non-profit laboratories may obtain the Phoenix cells from either Allele Biotechnology or the National Gene Vector Bank. The vectors may be obtained from Addgene. We are no longer distributing these reagents from the lab.

The following Cell Lines may be purchased from The National Gene Vector Laboratory Biorepository (NGVB)

Phoenix Ampho & Eco cell lines - second generation retroviral producer lines (MMULV-based).
PhoenixGP cell line, 293T-derived containing only gag-pol for VSV-G and other pseudotyping.
HEK293T cell line, a human cell line that was derived from the HEK293 cell line by the addition of the SV40 large T antigen. The cell line that can be transfected with plasmid DNA at high efficiency and commonly used for production of retroviral, lentiviral and other viral vectors using the transient transfection method.

The following Phoenix Cell Lines may also be purchased from Allele Biotechnology

Phoenix Ampho & Eco cell lines - second generation retroviral producer lines (MMULV-based).

The following vectors have been deposited at Addgene. Please search their online catalogs for information on obtaining these plasmids.

pCI-VSVG - VSV-G expression plasmid.
pBMN-LacZ vector - a pBabeMN based retroviral construct with the LacZ gene.

pBMN-i-EGFP vector - a pBabeMN based retroviral construct with the EMCV IRES upstream from the humanized/enhanced GFP (EGFP).
pBMN-z-i-neo vector - a pBabeMN based retroviral construct with the EMCV IRES upstream from the Neomycin resistance gene.
Felix vectors (pCPRdEnv; pLionII; pLionIIp-YFP; pTiger and pTiger-YFP) - FIV-based (Feline Immunodeficiency) vectors for delivery to non-dividing cells.