Osmotic Strength
Set up test tubes with about a half an inch of each of the following solutions:
CONTROL |
100% seawater |
90 |
9 parts seawater and 1 part distilled water |
80 |
8 parts seawater and 2 parts distilled water |
70 |
7 parts seawater and 3 part distilled water |
60 |
6 parts seawater and 4 part distilled water |
50 |
Equal parts seawater and distilled water |
- Add one drop of concentrated eggs to each.
- Swirl and let sit for one minute.
- Add one drop of diluted sperm suspension and swirl briefly.
- Wait 5 minutes.
- Score eggs for fertilization. If time permits can also score for later development [Next period see if divided. Next day see if there are swimming blastula.]