| An Initial
Comparison of Plasmid-Rescued Transposed RescueMu Flanking
Maize DNA with Highly Similara Sequences in Genbank
• RescueMu, similar to other active MuDR/Mu elements shows a strong preference for insertion into genes, not repetitive DNA. • For methodology and details, this table is an expanded version of Table 2 from Raizada, M.N., Nan, G.-L., and Walbot, V. (2001) Somatic and germinal mobility of the RescueMu transposon in transgenic maize. Plant Cell (submitted) located online at: http://www.plantcell.org • During Summer 2001, sequences for all of these insertions will be available and submitted to Genbank. Unfortunately, neither clones nor seed for these insertions are available at the present time. See http://zmdb.iastate.edu for more information on RescueMu sequences. • Genbank sequence similarity searches were performed between August 16 – September 2, 2000. • Blast searches were performed using the NR, dbEST and Swissprot databases at: http://www.ncbi.nlm.nih.gov/BLAST/ |
| Clone
and Border |
Somaticb
or Germinal |
Plant
Source |
#bpc |
Genbank Nucleotide (BlastN) Similarity | Genbank Protein (BlastX) Similarity | ||||||||
| |
Name |
Similarity
Region |
E Value | Similarity
Distance from Mud |
Matchf |
Name |
Similarity
Region |
E value | Similarity
Distance From Mu |
||||
| I-1R | S(1) | MrG157.2 | 196 | Z.mays
endosperm 605 cDNA Zantedeschia aethiopica ascorbate peroxidase cDNA |
gb/A1812163.1
gb/AF159254.1 |
13-205
89-135 |
2e-98
0.005 |
+1
+118 |
A.thaliana ascorbate peroxidase | emb/CAA06823.1 | aa 5-38 | 9e-06 | +1 |
| I-1L | S(1) | MrG157.2 | 220 | Neurospora crassa transcription factor (nuc-1+) | gb/M37700.1 | 630-661 | 6e-06 | +173 | N.S. | ||||
| I-2L | S(1) | MrG157.2 | 230 | S. bicolor pathogen-induced cDNA | gb/BE365114.1 | 68-99 | 6e-06 | +48 | N.S. | ||||
| I-4L | S(1) | MrG158.2 | 338 | Z.mays Irma + En1 element | emb/X72327.1 | 1582-1706 | 4e-05 | +97 | N.S. | ||||
| I-6L | S(1) | MrG158.2 | 236 | Wheat pre-anthesis spike cDNA | gb/BE500162.1 | 7-98 | 3e-20 | >+145 | A.thaliana putative aldolase | pir/T01902 | aa 87-137 | 3e-17 | >+82 |
| I-8L | S(1) | MrG158.2 | 537 | Z.mays ear tissue 606 cDNA | gb/A1691294.1 | 328-264 | 4e-06 | >+465 | Z.mays anther/pollen 660 cDNA ORF | gb/AW499237.1 | nt 217-49 | 4e-04 | >+398 |
| I-9R | S(1) | MrG158.2 | 375 | Z.mays mixed adult 707 cDNA | gb/AW399991.1 | 2-254 | 1e-114 | +57 | Z.mays mixed adult tissue 707 cDNA ORF | gb/AW39999.1 | nt 3-253 | 6e-37 | +58 |
| I-12L | S(1) | MrG157.2 | 396 | N.S. | S. bicolor pathogen-induced cDNA ORF | gb/BE364650.1 | nt 45-485 | 3e-04 | +5 | ||||
| I-13L | S(1) | MrG157.2 | 856 | N.S. | A.thaliana hypothetical protein T18E12.12 | pir/IT02705 | aa 257-304 | 3e-04 | >+713 | ||||
| I-14R | S(1) | MrG157.2 | 206 | N.S. | A.thaliana hypothetical protein | gb/AAD3924.1 | aa 153-194 | 4e-04 | +44 | ||||
| I-15L | S(1) | MrG157.2 | 363 | Z.mays ear tissue 606 cDNA | gb/A1854983.1 | 83-25 | 4e-17 | +259 | O.sativa
cDNA 5’ ORF Z.mays ear tissue 606 cDNA ORF |
gb/BE040029.1
gb/A1854983.1 |
nt 392-594
nt 93-19 |
1e-06
2e-06 |
+4
+249 |
| I-16R | S(1) | MrG157.2 | 179 | N.S. | Saccaromyces cerevisiae rad16 | pir/S25366 | aa 247-289 | 0.039 | +26 | ||||
| I-16L | S(1) | MrG157.2 | 561 | N.S. | Schizo
saccaromyces pombe rad16 homolog Medicago truncatula stem cDNA ORF |
pir/T40569
gb/AW695617.1 |
aa 378-417
nt 131-316 |
5e-08
5e-11 |
+146
+134 |
||||
| I-19L | S(1) | MrG157.2 | 627 | Z.mays
leaf primordium 486 cDNA Z.mays PHYT1 acidic phytase |
gb/A1657360.1
emb/AJ223470.1 |
347-176
1105-1269 |
1e-24
4e-34 |
+337
+337 |
Z.mays leaf primordia 486 cDNA ORF | gb/A1657360.1 | nt 219-347 | 3e-10 | +337 |
| I-20L | S(1) | MrG158.2 | 565 | N.S. | A.thaliana hypothetical protein F18F4.110 | pir/T04885 | aa 84-139 | 8e-04 | +301 | ||||
| I-20R | S(1) | MrG158.2 | 580 | Z.mays 14day embryo 683 cDNA | gb/AW067036.1 | 226-269 | 2e-05 | >+408 | N.S. | ||||
| I-21R | S(1) | MrG158.6 | 245 | Mu1terminal
inverted repeat S. bicolor dark grown tissue cDNA |
emb/X00913.1
gb/BE361626.1 |
152-14
145-191 |
4e-72
2e-05 |
+1-139
+170 |
Glycine max hypothetical glucose-regulated repressor (GRR1) | Gb/AAB70660.1 | aa 232-265 | 4e-05 | +143 |
| I-22L | S(1) | MrG157.2 | 544 | N.S. | Solanum tuberosum stolon cDNA ORF | gb/BE341870.1 | nt 222-350 | 4e-04 | +342 | ||||
| I-28R | S(1) | MrGH108-148 | 622 | Z.mays AdhI flanking region | gb/AF123535.1 | 131667-131428 | 2e-17 | >+436 | N.S. | ||||
| I-29R | S(1) | MrGH108-148 | 514 | O. sativa green shoot cDNA | dbj/AU096566.1 | 672-499 | 5e-33 | >+325 | A.thaliana
glucose-regulated repressor S. bicolor dark-grown tissue cDNA ORF |
gb/AAD20708.1
gb/BE361626.1 |
aa 182-348
nt 115-582 |
1e-50
5e-80 |
>+1
>+1 |
| I-30R | S(1) | MrGH108-148 | 433 | Z. mays14day embryo 683 cDNA | gb/AW067036.1 | 226-269 | 1e-05 | >+234 | N.S. | ||||
| I-32L | S(1) | MrGH108-148 | 420 | Z.mays
CAB48 binding protein Z.mays Irma+En1 element |
emb/X63205.1
emb/X72327.1 |
1037-1198
1582-1706 |
1e-05
5e-05 |
>+148
>+55-179 |
Z.mays tassel 618 cDNA ORF | gb/AW267299.1 | nt 351-515 | 3e-07 | >+217 |
| I-33R | S(1) | MrGH108-148 | 587 | Z. mays mixed tissue 945 cDNA | gb/AW927853.1 | 159-106 | 1e-06 | >+474 | Z.mays mixed adult tissue 707 cDNA ORF | gb/AW399951.1 | nt 100-20 | 6e-04 | >+474 |
| I-34R | S(1) | MrGH108-148 | 598 | Z. mays endosperm 605 cDNA | gb/A1795518.1 | 162-204 | 4e-12 | >+162 | Phalaenopsis
cysteine proteinase Z.mays endosperm 605 cDNA ORF |
gb/AAB37233.1
gb/A1795518.1 |
aa 26-75
nt 56-136 |
4e-09
8e-10 |
>+420
>+125 |
| I-36R | S(1) | MrGH108-148 | 513 | N.S. | mudrA protein | pir/S59141 | aa 455-489 | 3e-12 | >+115 | ||||
| I-38R | S(1) | MrGH110.94 | 376 | Z. maysimmature ear cDNA | gb/A1065431.1 | 102-157 | 2e-10 | >+206 | Z.mays inflorescence immature ear cDNA ORF | gb/A1065431.1 | nt 276-14 | 8e-08 | >+98 |
| I-39R | S(1) | MrGH110.94 | 567 | P.cinnamomi GRP78/BiP gene | emb/X75673.1 | 2371-2768 | 8e-17 | >+169 | Glycine max dnaK chaperonin BiP-B | pir/TO6358 | aa 245-420 | 4e-75 | >+39 |
| I-40R | S(1) | MrGH110.94 | 614 | O.sativa chromosome 1 DNA | dbj/AP001633.2 | 62221-62030 | 1e-15 | >+190 | A.thaliana putative lipase | dbj/BAA94236.1 | aa 249-363 | 3e-43 | >+186 |
| I-41R | S(1) | MrGH110.94 | 558 | Z. mays
ear tissue 606 cDNA Z.mays AdhI-Cm flanking DNA |
gb/A1691294.1
emb/X17556.1 |
340-171
5033-4847 |
3e-59
2e-29 |
>+261
>+259 |
Z.maysear tissue 606 cDNA ORF | gb/A1691294.1 | nt 340-146 | 2e-20 | >+261 |
| I-42R | S(1) | MrGH148.2 | 611 | Z. mays leaf primordia 486 cDNA | gb/A1622241.1 | 178-28 | 1e-64 | >+121 | Z.mays leaf primordia 486 cDNA ORF | gb/A1622241.1 | nt 19-297 | 4e-48 | >+2 |
| I-43R | S(1) | MrGH110.70 | 609 | Z. mays acetohydroxyacid synthase | emb/X63553.1 | 105-11 | 1e-12 | >+385 | N.S. | ||||
| I-44R | S(1) | MrGH148.2 | 325 | S. bicolordark grown cDNA | gb/BE361714.1 | 389-275 | 3e-36 | >+4 | Cicer arietinum
hypothetical protein S. bicolor dark grown tissue cDNA ORF |
emb/CAB95829.1
gb/BE361503.1 |
aa 398-436
nt 354-470 |
6e-16
3e-36 |
>+3
>+3 |
| I-45R | S(1) | MrGH110.70 | 511 | N.S. | A.thalianaputative protein kinase | gb/AAC23760.1 | aa 79-243 | 3e-06 | >+1 | ||||
| I-52R | G(2) | SH-C9.40 | 656 | Z.mays root 614 cDNA | gb/AW053014.1 | 2-138 | 2e-70 | +57 | A.thaliana
membrane carrier protein Z.mays root 614 cDNA ORF |
gb/AAF27035.1
gb/AW053014.1 |
aa 2-49
nt 4-138 |
4e-12
2e-21 |
+47
+59 |
| I-53L | NDe(1) | SH-C15.19 | 340 | N.S. | Z.mays tassel primordium 946 cDNA ORF | gb/BE238649.1 | nt 13-102 | 2e-05 | >+11 | ||||
| I-54L | G(4) | SH4712 | 589 | N.S. | H.sapiens cDNA ORF | gb/BE277167.1 | nt 232-573 | 5e-08 | +245 | ||||
| I-55L | G(2) | SH4713 | 693 | O.sativaflowering panicle cDNA | dbj/C98637.2 | 432-295 | 2e-23 | +165 | A.thaliana
unknown protein O.sativa flowering panicle cDNA ORF |
dbj/BAB01173.1
dbj/C98637.2 |
aa 258-307
nt 431-285 |
3e-05
8e-33 |
+181
+166 |
| I-55R | G(2) | SH4713 | 653 | O.sativaflowering panicle cDNA | dbj/C72506.1 | 276-337 | 2e-11 | +249 | O.sativa flowering panicle cDNA ORF | dbj/C72506.1 | nt 27-155 | 2e-25 | +3 |
| I-56L | G(2) | VC-M807 | 536 | Loligo opalescens voltage-dependent K+ channel cDNA | gb/U50543.1 | 1771-1236 | 0 | >+1 | Loligo opalescens voltage-dependent K+ channel SqKv1A | gb/AAB02884.1 | aa 255-418 | 2e-69 | >+8 |
| I-57L | G(2) | VC -E10-4B.1 | 582 | S. bicolorwater-stressed cDNA | gb/AW925097.1 | 27-188 | 3e-25 | +419 | S. bicolor water-stressed cDNA ORF | gb/AW679244.1 | nt 171-64 | 4e-09 | +426 |
| I-57R | G(2) | VC-E10-4B.1 | 666 | Z.mays endosperm 605 cDNA | gb/A1665158.1 | 448-243 | 1e-107 | +400 | A.thaliana
unknown protein Z.mays endosperm 605 cDNA ORF |
dbj/BAA97034.1
gb/AI665158.1 |
aa 73-160
nt 541-260 |
2e-39
7e-57 |
+224
+215 |
| I-58L | G(2) | VC-M833.7 | 155 | Z.mays root 614 cDNA | gb/AW000292.1 | 15-151 | 8e-66 | +19 | A.thaliana unknown protein | gb/AAF88114.1 | aa 1-56 | 2e-05 | +145 |
| I-58R | G(2) | VC-M833.7 | 424 | Z.mays
maize embryo 687 cDNA Z.mays inverted repeat element |
gb/AW061911.1
gb/AF203732.1 |
225-177
8-225 |
5e-11
8e-10 |
+213
+225 |
Z.mays early embryo 687 cDNA ORF | gb/AW061911.1 | nt 116-244 | 1e-07 | +194 |
| I-59L | G(7) | VC-M835 B3 | 671 | Z.mays
leaf primordia 486 cDNA Z.mays inverted repeat element |
gb/A1467722.1
gb/AF203733.1 |
384-283
8-225 |
1e-18
8e-10 |
+240
+226 |
Z.mays leaf primordia 486 cDNA ORF | gb/A1444709.2 | nt 300-542 | 6e-17 | +118 |
| I-59R | G(7) | VC-M835 B3 | 646 | Z.mays
root 614 CDNA Z.mays PREM-IE retroelement |
gb/AW126419.1
gb/UO3684.1 |
447-615
1367-1633 |
9e-66
1e-110 |
+185
+185 |
A.thaliana
unknown protein S. bicolor water-stressed cDNA ORF |
dbj/BAA97477.1
gb/AW924018.1 |
aa 1-75
nt 98-334 |
1e-11
4e-32 |
+88
+85 |
| I-62R | NDe(1) | VC-M821.5 | 458 | Wheat etiolated seedling root cDNA | gb/BE446702.1 | 228-359 | 7e-32 | +109 | A.thaliana
hypothetical protein Wheat etiolated seedling root cDNA ORF |
gb/AAD41986.1
gb/BE446702.1 |
aa 175-252
nt 228-365 |
3e-17
1e-18 |
+109
+109 |
| I-63R | NDe(1) | VC-M835 B-3 | 459 | Wheat cold-stressed cDNA | gb/BE490667.1 | 370-396 | 5e-05 | +327 | N.S. | ||||
| I-64R | NDe(1) | VC-M835 B3 | 424 | N.S. | O. sativa
retrotransposon RIRE2 orf3 Z.mays ear tissue 606 cDNA ORF |
dbj/BAA84461.1
gb/AI770346.1 |
aa 45-119
nt 625-437 |
1e-04
1e-11 |
+139
+175 |
||||
| I-64L | NDe(1) | VC-M835 B3 | 279 | N.S. | Drosophila
Ariadne -2 zinc finger protein Wheat etiolated seedling root cDNA ORF |
gb/AJ010169
gb/BE442797.1 |
aa 332-416
nt 5-211 |
7e-09
5e-12 |
+2
+2 |
||||
| I-65R | NDe(1) | VC-M833.7 | 611 | Z.mays hox1a and trap genes | emb/AJ238507.1 | 4791-4999 | 7e-05 | >+160 | N.S. | ||||
| I-65L | NDe(1) | VC-M833.7 | 760 | Wheat unstressed
shoot cDNA Saccarum membrane protein cDNA |
gb/BE425841.1
gb/L13655.1 |
69-258
227-397 |
2e-45
4e-16 |
>+131
>+144 |
Saccharum
membrane protein O.sativa root cDNA ORF |
gb/AAA02747.1
dbj/AU065447.1 |
aa 32-96
nt 76-279 |
1e-19
9e-24 |
>+133
>+130 |
| I-66R | NDe(1) | VC-M833.7 | 296 | Rhizobium nodPQ (vector) | emb/Z14809.1 | 1983-1706 | 1e-141 | +1 | Rhizobium nodPQ | sp/P134421 | aa 125-220 | 5e-44 | +2- |
| I-66L | NDe(1) | VC-M833.7 | 549 | pBluescript vector | emb/X52326.1 | 693-278 | 0 | +135 | beta-galactosidase (vector) | gb/AAB05989.1 | aa 42-94 | 2e-19 | +135 |
| I-67R | NDe(1) | VC-M833.7 | 726 | Z.mays mixed adult 945 cDNA | gb/BE128939.1 | 174-24 | 2e-21 | >+469 | Z.mays mixed adult tissue 945 cDNA ORF | gb/BE128939.1 | nt 177-4 | 2e-09 | >+466 |
| I-68R | NDe(1) | VC-M833.7 | 704 | Z.mays anther/pollen 660 cDNA | gb/AW313235.1 | 347-1 | 1e-165 | >+1 | A.thaliana
putative esterase Z.mays leaf primordia 486 cDNA ORF |
db/AAD17422.1
gb/AI438579.2 |
aa 248-312
nt 300-1 |
8e-09
2e-56 |
>+44
>+76 |
| I-68L | NDe(1) | VC-M833.7 | 130 | Z.mays anther/pollen 660 cDNA | gb/AW313235.1 | 352-475 | 3e-28 | >+6 | Z.maysleaf primordia 486 cDNA ORF | gb/A1438579.3 | nt 483-376 | 2e-05 | >+2 |
| I-70R | G(8) | SD-B45.1/3 | 550 | N.S. |
|
O.sativablast
infection stage cDNA ORF Schizosaccaromyces pombe late log phage cDNA ORF |
gb/AW155307.1
dbj/AU010204.1 |
nt 68-217
nt 209-358 |
2e-07
3e-07 |
>+310
>+310 |
|||
| I-72R | G(6) | SD-B40.3 | 495 | Z.mays PREM2 retroelement gag | gb/U41000.1 | 6810-7213 | 0 | >+92 | Z. mays copia retroelement pol polyprotein | gb/AAD20307.1 | aa 897-1030 | 7e-71 | >+94 |
| I-74R | NDe(1) | SD-B45.3 | 554 | Z. mays tassel primordium cDNA | gb/BE238476.1 | 346-370 | 6e-05 | >+210 | N.S. | ||||
| I-75R | NDe(1) | VC-E7-3 | 588 | N.S. | Gossypium arboreum 7-10 dpa fiber ORF | gb/AW730508.1 | nt 642-797 | 3e-04 | +9 | ||||
| I-76L | NDe(1) | VC-E10-4 | 722 | Z.mays
ear tissue 606 cDNA S.bicolor dark grown tissue cDNA |
gb/AI770651
gb/BE359038.1 |
68-157
12-63 |
4e-06
3e-10 |
+52
+271 |
Triticum aestivum scutellum cDNA ORF | gb/BE419899.1 | nt 10-268 | 4e-11 | +3 |
| I-76R | NDe(1) | VC-E10-4 | 729 | Z.mays ear tissue 606 cDNA | gb/AI714482 | 52-434 | 6e-35 | +283 | Z.mays ear tissue 606 cDNA ORF | gb/AI714482.1 | nt 52-455 | 2e-33 | +275 |
| I-77R | NDe(1) | SH-C99-9-40 | 282 | Z.mays root 614 cDNA | gb/AW053014 | 2-147 | 1e-26 | +49 | A.thaliana unknown protein, weak similarity to yeast mitochondrial carrier protein | gb/AAF27035.1 | aa 2-49 | 2e-12 | +39 |
| I-79L | NDe(1) | SH-C99-15-8 | 291 | Z.mays endosperm 605 cDNA | gb/AI577130 | 237-422 | 9e-05 | +68 | Lotus japonicus seedling cDNA ORF | dbj/AV419453.1 | nt 53-178 | 2e-04 | +59 |
| I-80L | NDe(1) | SH-C99-15-8 | 653 | N.S. | Hordeum vulgare pre-anthesis spike ORF | gb/BE455449.1 | nt 130-497 | 3e-10 | +234 | ||||
| I-80R | NDe(1) | SH-C99-15-8 | 655 | N.S. | S.bicolor water-stressed cDNA ORF | gb/AW680321.1 | nt 9-129 | 7e-04 | +336 | ||||
| I-82L | NDe(1) | SH-C99-15-19 | 320 | Z.mays early embryo 687 cDNA | gb/AW224909 | 37-163 | 0.0002 | +23 | Z.mays tassel primordium cDNA ORF | gb/BE238649.1 | nt 13-102 | 8e-06 | +11 |
| I-83L | NDe(1) | SH4712 | 743 | Z.mays early embryo 687 cDNA | gb/AW257861 | 3-201 | 4e-29 | +541 | A.thaliana putative CorA-like Mg2+ transporter | gb/AAF14678.1 | aa 45-94 | 1e-05 | +277 |
| I-83R | NDe(1) | SH4712 | 766 | Z.mays ear tissue 606 cDNA | gb/AI881975 | 1-289 | 8e-12 | +20 | Z.mays copia-type pol protein | gb/AAD20307.1 | aa 599-663 | 2e-30 | +445 |
| I-84L | NDe(1) | SH4713 | 709 | O.sativa flowering panicle cDNA | dbj/C98637.2 | 91-267 | 7e-06 | +204 | O.sativa flowering panicle cDNA ORF | dbj/C98637.2 | nt 320-28 | 3e-09 | +143 |
| I-84R | NDe(1) | SH4713 | 723 | S.bicolor pathogen-induced cDNA | gb/BE596140.1 | 51-525 | 2e-77 | +162 | S.bicolorpathogen-induced cDNA ORF | gb/BE596140.1 | nt 8-524 | 4e-64 | +128 |
| I-85L | NDe(1) | SH4713 | 723 | Z.mays mixed adult 707 cDNA | gb/AW330935 | 124-259 | 6e-14 | +210 | A.thaliana putative protein | gb/AAF79266.1 | aa 17-58 | 6e-09 | +217 |
| I-85R | NDe(1) | SH4713 | 671 | Z.mays anther/pollen 660 cDNA | gb/AW520130 | 46-111 | 2e-05 | +605 | N.S. | ||||
| I-86L | NDe(1) | GN 673005A1 | 498 | Z.mays mixed adult 707 cDNA | gb/AW324597 | 1-64 | 9e-07 | +25 | Z.mays Ca2+-dependent protein kinase | pir/T03023 | aa 51-183 | 4e-48 | +66 |
| I-87L | NDe(1) | GN673005A2 | 506 | Triticum aestivum heat-stressed seedling cDNA | gb/BE496991.1 | 476-510 | 2e-07 | +119 | A.thaliana pherophorin-like protein | emb/CAA16736.1 | aa 425-540 | 3e-28 | +3 |
| I-87R | NDe(1) | GN673005A2 | 543 | Triticum aestivum heat-stressed seedling cDNA | gb/BE496991.1 | 479-344 | 6e-39 | +97 | A.thalianapherophorin-like protein | emb/CAA16736.1 | aa 392-440 | 2e-12 | +94 |
| I-89L | NDe(1) | GN673005B1 | 531 | Hordeum vulgare pre-anthesis spike cDNA | gb/BE456076.1 | 111-179 | 2e-07 | +148 | N.S. | ||||
| I-89R | NDe(1) | GN673005B1 | 566 | N.S. | Hordeum vulgare 5-45 DAP spike cDNA ORF | gb/BE455089.1 | nt 1210-847 | 8e-04 | +30 | ||||
| I-90R | NDe(1) | GN6730005C1 | 810 | Z.mays immature ear cDNA | gb/AI065431 | 8-326 | 2e-21 | +259 | Z.mays immature ear cDNA ORF | gb/AI065431.1 | nt 314-14 | 9e-05 | +245 |
| I-92R | NDe(1) | VC-M833.7 | 762 | N.S. | A.thaliana hypothetical protein | gb/AAF63640.1 | aa 3-54 | 4e-09 | +133 | ||||
Abbreviations: R, right border; L, left border; S, somatic; G, germinal; ND, not determined; N.S., not significant similarity; ORF, open reading frame; Z.mays, Zea mays; O.sativa, Oryza sativa; A.thaliana, Arabidopsis thaliana; S. bicolor, Sorghum bicolor; H.Sapiens, Homo sapiens; nt, nucleotide; aa, amino acid. aHigh sequence similarity arbitrarily defined as E?1e-03 bThe number in parenthesis refers to the number of colonies in which the allele was recovered from a single plasmid rescue. A germinal insertion was confirmed by Southern blot inheritance and/or by multiple allele recoveries. cRefers to the number of nucleotides sequenced. dThe greater (>) symbol is used when the 9 bp target site duplication was not sequenced. The precise location of the RescueMu insertion is not known. eThis plant carried germinal RescueMu insertions by Southern blot, but the allele was not tested for transmission to the progeny. fWhere an ORF is specified, this match is to the tblastX translation of the cDNA. |