MaxChelator References

Most recent reference:

Patton, Thompson and Epel.
"Some precautions in using chelators to buffer metals in biological solutions."
Cell Calcium. 35/5 pp. 427-431, 2004.

PDF copy available upon request: cpatton@stanford.edu

An earlier paper [pre PDF]:

Methods Cell Biol. 1994; 40:3-29, Academic Press; San Diego.
A Practical Guide to the Study of Ca2+ in Living Cells

Chapter is:
A Practical Guide to the Preparation of Ca Buffers.
Donald Bers, Chris Patton, and Richard Nuccitelli

The programs change. Always state which version of the programs you are using.

I would appreciate it if you would mention the URL to MAXC: maxchelator.stanford.edu

Papers used to help build the MAXC programs:

1) Bers, D.M. A simple method for the accurate determination of free [Ca] in Ca-EGTA solutions. Am. J. Physiol., 242:C404-C408, 1982.

2) Brook & Storey, Analytical Biochemistry 201. 119-126 (1992), Bound and Determined: A computer program for making buffers of defined Ion Concentrations. [see downloads section to retrieve a copy of this program, BAD]

3) Fabiato & Fabiato, J. Physiol, Paris, 1979, 75, 463-505, Calculator programs for computing the composition of solutions containing multiple metals and ligands used for experiments in skinned muscle cells.

4) Harrison & Bers, Biochimica et Biophysica Acta 925 (1987) 133-143, The effect of temperature and ionic strength on the apparent Ca-affinity of EGTA and the analogous Ca-chelators BAPTA and dibromo-BAPTA.

5) Harrison, S.M. and D.M. Bers. Correction of absolute stability constants of EGTA for temperature and ionic strength. Am. J. Physiol. 256:C1250-C1256, 1989.

6) Martell and Smith, Critical Stability Constants, 1974 for volume 1 now up to volume 6?

7) Good, Biochemistry, 1966, 5, 467-477, Hydrogen Ion Buffers for Biological Research.

8) Schoenmakers, Visser, Flik and Theuvenet, BioTechniques v12, #6 (1992) 870-879, CHELATOR: An Improved Method for Computing Metal Ion Concentrations in Physiological Solutions. [see downloads section to retrieve a copy of this program]

9) McGuigan, Kay, Elder and Luthi, Magnesium Research (2007 ) 20(1)72-81, Comparison between measured and calculated ionised concentrations in Mg2+/ATP, Mg2+/EDTA and Ca2+/EGTA buffers; influence pf changes in temperature, pH and pipetting errors on the ionised concentrations.

I can be reached by email at: cpatton@stanford.edu for questions, comments, problems, etc.