MAGNESIUM
See: http://probes.invitrogen.com/handbook/sections/1900.html
They have been adapted to the MAXC format assuming that, being derived from BAPTA, the hydrogen disassociation constants are the same (admittedly a "wild" assumption, but we have to start somewhere?).
In order of increasing Mg affinity:
|
|
CALCIUM |
MAGNESIUM |
||
|
CHELATOR |
Kd (nM) |
MAXC |
Kd (mM) |
MAXC |
|
BAPTA |
136 |
6.970 |
22.5 |
1.77 |
|
Mag-Orange |
12,000 |
3.02 |
3.9 |
2.53 |
|
Mag-Indo-1 |
? |
? |
2.7 |
2.69 |
|
Mag-Fura-Red |
17,000 |
2.87 |
2.5 |
2.72 |
|
Mag-Fura-5 |
28,000 |
2.65 |
2.3 |
2.76 |
|
Mag-Fura-2 |
25,000 |
2.705 |
1.9 |
2.84 |
|
Mag-Green |
6,000 |
3.325 |
1.0 |
3.12 |
The rest of the MAXC constants for BAPTA are: [set H4 to ZERO for most of the indicators above]
|
H1 |
H2 |
H3 |
H4 |
|
6.360 |
5.470 |
1.000 |
1.000 |
|
|
|
|
|
|
dH1 |
dH2 |
dH3 |
dH4 |
|
0.01 |
0.01 |
0.00 |
0.00 |
The Ca-BAPTA and Mg-BAPTA constants are:
|
CaM |
CaHM |
|
MgM |
MgHM |
|
6.970 |
? |
|
1.770 |
? |
|
|
|
|
|
|
|
dCaM |
dCaHM |
|
dMgM |
dMgHM |
|
4.70 |
? |
|
? |
? |
If anyone has more precise values for any of the above, please let me know, cpatton@stanford.edu
These values are more likely to be valid the closer they are to the experimental conditions that Molecular Probes used to assess the Kd in the first place, 22C, pH 7.2, 0.110N