Selected Publications

• Woodard LE, Hillman RT, Keravala A, Lee S, Calos MP. (2009). Effect of nuclear localization and hydrodynamic delivery-induced cell division on phiC31 integrase activity. Gene Ther. 2009 Oct 22. [Epub ahead of print][Abstract]
  
  
• Keravala A, Ormerod BK, Palmer TD, Calos MP. (2008). Long-term transgene expression in mouse neural progenitor cells modified with phiC31 integrase. J Neurosci Methods. 173(2):299-305. [Abstract]
  
  
• Keravala A, Calos MP. (2008). Site-specific chromosomal integration mediated by phiC31 integrase. Methods Mol Biol. 435:165-73. [Abstract]
  
  
• Fish MP, Groth AC, Calos MP, Nusse R. (2007). Creating transgenic Drosophila by microinjecting the site-specific phiC31 integrase mRNA and a transgene-containing donor plasmid. Nat Protoc. 2(10):2325-31. [Abstract]
  
  
• Calos MP. (2007). The phiC31 integrase system for gene therapy. Curr Gene Ther. 6(6):633-45. [Abstract]
  
  
• Portlock JL, Keravala A, Bertoni C, Lee S, Rando TA, Calos MP. (2006). Long-term increase in mVEGF164 in mouse hindlimb muscle mediated by phage phiC31 integrase after nonviral DNA delivery. Hum Gene Ther. 17(8):871-6. [Abstract]
  
  
• Chalberg TW, Vankov A, Molnar FE, Butterwick AF, Huie P, Calos MP, Palanker DV. (2006). Gene transfer to rabbit retina with electron avalanche transfection. Invest Ophthalmol Vis Sci. 47(9):4083-90. [Abstract]
  
  
• Keravala A, Portlock JL, Nash JA, Vitrant DG, Robbins PD, Calos MP. (2006). PhiC31 integrase mediates integration in cultured synovial cells and enhances gene expression in rabbit joints. J Gene Med. 8(8):1008-17. [Abstract]
  
  
• Keravala A, Groth AC, Jarrahian S, Thyagarajan B, Hoyt JJ, Kirby PJ, Calos MP. (2006). A diversity of serine phage integrases mediate site-specific recombination in mammalian cells. Mol Genet Genomics. 276(2):135-46. [Abstract]
  
  
• Ishikawa Y, Tanaka N, Murakami K, Uchiyama T, Kumaki S, Tsuchiya S, Kugoh H, Oshimura M, Calos MP, Sugamura K. (2006). Phage phiC31 integrase-mediated genomic integration of the common cytokine receptor gamma chain in human T-cell lines. J Gene Med. 8(5):646-53. [Abstract]
  
  
• Chalberg TW, Portlock JL, Olivares EC, Thyagarajan B, Kirby PJ, Hillman RT, Hoelters J, Calos MP. (2006). Integration specificity of phage phiC31 integrase in the human genome. J Mol Biol. 357(1):28-48. [Abstract]
  
  
• Bertoni C, Jarrahian S, Wheeler TM, Li Y, Olivares EC, Calos MP, Rando TA. (2006). Enhancement of plasmid-mediated gene therapy for muscular dystrophy by directed plasmid integration. Proc Natl Acad Sci U S A. 103(2):419-24. [Abstract]
  
  
• Ginsburg DS, Calos MP. (2005). Site-specific integration with phiC31 integrase for prolonged expression of therapeutic genes. Adv Genet. 54:179-87. [Abstract]
  
  
• Thyagarajan B, Calos MP. (2005). Site-specific integration for high-level protein production in mammalian cells. Methods Mol Biol. 308:99-106. [Abstract]
  
  
• Chalberg TW, Genise HL, Vollrath D, Calos MP. (2005). phiC31 integrase confers genomic integration and long-term transgene expression in rat retina. Invest Ophthalmol Vis Sci. 46(6):2140-6. [Abstract]
  
  
• Held PK, Olivares EC, Aguilar CP, Finegold M, Calos MP, Grompe M. (2005). In vivo correction of murine hereditary tyrosinemia type I by phiC31 integrase-mediated gene delivery. Mol Ther. 11(3):399-408. [Abstract]
  
  
• Quenneville SP, Chapdelaine P, Rousseau J, Beaulieu J, Caron NJ, Skuk D, Mills P, Olivares EC, Calos MP, Tremblay JP. (2004). Nucleofection of muscle-derived stem cells and myoblasts with phiC31 integrase: stable expression of a full-length-dystrophin fusion gene by human myoblasts. Mol Ther. 10(4):679-87. [Abstract]
  
  
• Dorigo O, Gil JS, Gallaher SD, Tan BT, Castro MG, Lowenstein PR, Calos MP, Berk AJ. (2004). Development of a novel helper-dependent adenovirus-Epstein-Barr virus hybrid system for the stable transformation of mammalian cells. J Virol. 78(12):6556-66. [Abstract]
  
  
• Groth AC, Fish M, Nusse R, Calos MP. (2004). Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Genetics. 166(4):1775-82. [Abstract]
  
  
• Groth AC, Calos MP. (2004). Phage integrases: biology and applications. J Mol Biol. 335(3):667-78. [Abstract]
  
  
• Hollis RP, Stoll SM, Sclimenti CR, Lin J, Chen-Tsai Y, Calos MP. (2003). Phage integrases for the construction and manipulation of transgenic mammals. Reprod Biol Endocrinol. 1:79. [Abstract]
  
  
• Portlock JL, Calos MP. (2003). Site-specific genomic strategies for gene therapy. Curr Opin Mol Ther. 5(4):376-82. [Abstract]
  
  
• Ortiz-Urda S, Thyagarajan B, Keene DR, Lin Q, Calos MP, Khavari PA. (2003). PhiC31 integrase-mediated nonviral genetic correction of junctional epidermolysis bullosa. Hum Gene Ther. 14(9):923-8. [Abstract]
  
  
• Olivares EC, Hollis RP, Chalberg TW, Meuse L, Kay MA, Calos MP. (2002). Site-specific genomic integration produces therapeutic Factor IX levels in mice. Nat Biotechnol. 20(11):1124-8. [Abstract]
  
  
• Ortiz-Urda S, Thyagarajan B, Keene DR, Lin Q, Fang M, Calos MP, Khavari PA. (2002). Stable nonviral genetic correction of inherited human skin disease. Nat Med. 8(10):1166-70. [Abstract]
  
  
• Stoll SM, Ginsburg DS, Calos MP. (2002). Phage TP901-1 site-specific integrase functions in human cells. J Bacteriol. 184(13):3657-63. [Abstract]
  
  
• Sclimenti CR, Thyagarajan B, Calos MP. (2001). Directed evolution of a recombinase for improved genomic integration at a native human sequence. Nucleic Acids Res. 29(24):5044-51. [Abstract]
  
  
• Olivares EC, Hollis RP, Calos MP. (2001). Phage R4 integrase mediates site-specific integration in human cells. Gene. 278(1-2):167-76. [Abstract]
  
  
• Thyagarajan B, Olivares EC, Hollis RP, Ginsburg DS, Calos MP. (2001). Site-specific genomic integration in mammalian cells mediated by phage phiC31 integrase. Mol Cell Biol. 21(12):3926-34. [Abstract]
  
  
  

Go to the Top of the Page.