phiC31 integrase in liver  
Stanford University  
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Michele Calos

Michele Pamela Calos

Professor
Department of Genetics

Publications

  1.  Calos, M.P. (2016). The CRISPR way to think about Duchenne’s. New England Journal of Medicine 374, 1684-1686.
  2.  Turan, S., Farruggio, A.P., Srifa, W., Day, J.W., and Calos, M.P. (2016). Precise correction of disease mutations in induced pluripotent stem cells derived from patients with limb girdle muscular dystrophy. Molecular Therapy 24, 685-696.
  3.  Calos, M.P. (2016). Phage integrases for genome editing. In Genome Editing: The Next Step in Gene Therapy. Eds. Cathomen, T., Hirsch, M., Porteus, M., ASGCT and Springer. Advances in Experimental Medicine and Biology 895, 81-91, DOI 10.1007/978-1-4939-3509-3_5.
  4.  Geisinger, J.M., Turan, S., Hernandez, S., Spector, L.P., and Calos, M.P. (2016). In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining. Nucleic Acids Research 44 (8): e76.
  5.  Woodard, L.E. and Michele P. Calos (2015). Nonviral genome modification strategies for gene therapy: Transposon, integrase, and nuclease systems, Chapter 26, in Gene and Cell Therapy Therapeutic Mechanisms and Strategies, Fourth Edition, ed. Templeton, N.S., CRC Press, pp.675-700.
  6.  Geisinger, J.M. and Calos, M.P. (2015). Using phage integrases in a site-specific dual integrase cassette exchange strategy. Methods Mol. Biol. 1239, 29-38
  7.  Farruggio, A.P. and Calos, M.P. (2014) Serine integrase chimeras with activity in E. coli and HeLa cells. Biology Open 3, 895-903. PMID 25217617
  8.  Zhao, C., Farruggio, A.P., Bjornson, C.R.R., Chavez, C.L., Geisinger, J.M., Neal, T.L., Karow, M, and Calos, M.P. (2014). Recombinase-mediated reprogramming and dystrophin gene addition in mdx mouse induced pluripotent stem cells. PLoS ONE 9, e96279.
  9.  Zhu, F., Gamboa, M., Farruggio, A.P., Hippenmeyer, S., Tasic, B., Schuele, B., Chen-Tsai, Y., and Calos, M.P. (2014). DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Nucleic Acids Research 42, e34. PMID 24304893
  10.  Tremblay JP, Xiao X, Aartsma-Rus A, Barbas C, Blau HM, Bogdanove AJ, Boycott K, Braun S, Breakefield XO, Buren JA, Buschmann M, Byrne BJ, Calos M, et al. (2013) Translating the genomics revolution: the need for an international gene therapy consortium for monogenic diseases. Mol Ther. 21, 266-268.
  11.  Geisinger, J.M. and Calos, M.P. (2013). Site-specific recombination using phiC31 integrase. In “Site-directed insertion of transgenes.”, P. Duchateau and S. Renault (eds.), Springer, Topics in Current Genetics 23, Chapter 8, pp.211 - 239.
  12.  Farruggio, A.P., Chavez, C.L., Mikell, C.L., and Calos, M.P. (2012). Efficient reversal of phiC31 integrase recombination in mammalian cells. Biotechnol. J. 7, 1332 - 1336. PMID22933343.
  13.  Lan, F., Liu, J., Narsinh, K., Hu, S., Han, L., Lee, A.S., Karow, M., Nguyen, P.K., Nag, D., Calos, M.P., Robbins, R.C., and Wu, J.C. (2012). Safe genetic modification of cardiac stem cells using a site-specific integration technique. Circulation 126 (suppl 1): S20 – S28.
  14.  Hillman RT, Calos MP. (2012) Site-specific integration with bacteriophage ΦC31 integrase. Cold Spring Harb Protoc; May:2012 (5).
  15.  Chavez, C.L., Keravala, A., Chu, J.N., Farruggio, A.P., Gabrovsky, V.E., Voorberg, J., and Calos, M.P. (2012). Long-term expression of human coagulation factor VIII in a tolerant mouse model using the phiC31 integrase system. Human Gene Therapy 23, 390 – 398.
  16.  Karow, M., Chavez, C.L., Farruggio, A.P., Geisinger, J.M., Keravala, A., Jung, W.E., Lan, F., Wu, J.C., Chen-Tsai, Y., and Calos, M.P. (2011) Site-specific recombinase strategy to create iPS cells efficiently with plasmid DNA. Stem Cells 29, 1696 - 1704.
  17.  Chavez, C.L. and Calos, M.P. (2011). Therapeutic applications of the phiC31 integrase system. Current Gene Therapy 11, 375 - 381.
  18.  Karow, M. and Calos, M.P. (2011). The therapeutic potential of phiC31 integrase as a gene therapy system. Expert Opin. Biol. Ther. 11, 1287-1296.
  19.  Keravala, A., Chavez, C.L., Hu, G., Woodard, L.E., Monahan, P.E., and Calos, M.P. (2011) Long-term phenotypic correction in factor IX knockout mice by using phiC31 integrase-mediated gene therapy. Gene Therapy 18, 842-848.
  20.  Ito, M., Yamanouchi, K., Naito, K., Calos, M.P. and Tojo, H. (2011). Site-specific integration of transgene targeting an endogenous lox-like site in early mouse embryos. J. Appl. Genet. 52, 89 -94.
  21.  Chavez, C.L., Keravala, A., Woodard, L.E., Hillman, R.T., Stowe, T.R., Chu, J.N., and Calos, M.P. (2010). Kinetics and longevity of C31 integrase in mouse liver and cultured cells. Human Gene Therapy 21, 1287 – 1297.
  22.  Woodard, L.E., Keravala, A., Jung, W.E., Wapinski, O., Yang, Q., Felsher, D.W., and Calos, M.P. (2010). Impact of hydrodynamic injection and phiC31 integrase on tumor latency in a mouse model of MYC-induced hepatocellular carcinoma. PLoS One, 5, e11367.
  23.  Woodard, L.E. and Calos, M.P. (2010). DNA integrating vectors (transposon, integrase). In A Guide to Human Gene Therapy, Chapter 8, (R.W. Herzog and S. Zolotukhin, eds.) World Scientific Publishing Co., pp. 123 - 138.
  24.  Woodard, L.E., Hillman, R.T., Keravala, A., Lee, S., and Calos, M.P. (2010). Effect of nuclear localization and hydrodynamic delivery-induced cell division on C31 integrase activity. Gene Therapy 17, 217-226.
  25.  Jung, W.E. and Calos, M.P. (2009). PhiC31 integrase for modification of stem cells. In Emerging Technology Platforms for Stem Cells, Chapter 20, (U. Lakshmipathy, J.D. Chesnut, and B. Thyagaragan, eds.) John Wiley and Sons, Inc.
  26.  Keravala, A., Lee, S., Thyagarajan, B., Olivares, E.C., Gabrovsky, V., Woodard, L.E. and Calos, M.P. (2009). Mutants of phiC31 integrase with enhanced efficiency and specificity. Molecular Therapy 17, 112-120.
  27.  Woodard, L.E. and Calos, M.P. (2008). Nonviral genome modification strategies for gene therapy: Transposon, integrase, and homologous recombination systems. In Gene and Cell Therapy: Therapeutic Mechanisms and Strategies, 3rd Edition. (N.S. Templeton, ed.) Taylor & Francis Group, CRC Press, Boca Raton, FL. Chapter 31, 677-691.
  28.  Gabrovsky, V. and Calos, M.P. (2008). Factoring nonviral gene therapy into a cure for hemophilia A. Current Opinion in Molecular Therapeutics 10, 464-470. (N.S. Templeton, ed.) Taylor & Francis Group, CRC Press, Boca Raton, FL. Chapter 31, 677-691.
  29.  Keravala, A., Ormond, B.K., Palmer, T.D., and Calos, M.P. (2008). Long-term transgene expression in mouse neural progenitor cells modified with C31 integrase. J. Neuroscience Meth. 173, 299-305.
  30.  Keravala, A. and Calos, M.P. (2007). Site-specific chromosomal integration mediated by C31 integrase. Methods in Molecular Biology 435: Chromosomal Mutagenesis. (G. D. Davis and K. J. Kayser, eds.) Humana Press, Totowa, NJ, Chapter 12, 165-173.
  31.  Hillman, R.T. and Calos, M.P. (2007). Site-specific integration with phage C31 integrase. In Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual. (T. Friedmann and J. Rossi, eds.) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. Chapter 65, 653 - 660.
  32.  Fish, M.P., Groth, A.C., Calos, M.P., and Nusse, R. (2007). Creating transgenic Drosophila by microinjecting the site-specific C31 integrase mRNA and a transgene-containing donor plasmid. Nature Protocols, doi:10.1038/nprot.2007.328.
  33.  Calos, M.P. (2006). The C31 integrase system for gene therapy. Current Gene Therapy 6, 633 - 645.
  34.  Portlock, J.L., Keravala, A., Bertoni, C., Lee, S., Rando, T., and Calos, M.P. (2006). Long-term increase in mVEGF164 in mouse hindlimb muscle mediated by phage C31 integrase after non-viral DNA delivery. Human Gene Therapy 17, 871 - 876.
  35.  Chalberg, T., Vankov, A., Molnar, F., Butterwick, A.F., Huie, P., Calos, M.P., and Palanker, D. (2006). Gene transfer to rabbit retina with electron-avalanche transfection. Investigative Ophthalmology & Visual Science 47, 4083 - 4090.
  36.  Keravala, A., Portlock, J.L., Nash, J., Robbins, P., and Calos, M.P. (2006). C31 integrase facilitates stable integration in synovial cells in vitro and enhances gene expression in vivo. J. Gene Med. 8, 1008 – 1017.
  37.  Keravala, A., Groth, A.C., Jarrahian, S., Hoyt, J.J., Thyagarajan, B.T., Kirby, P., and Calos, M.P. (2006). A diversity of serine phage integrases mediate site-specific recombination in mammalian cells. Mol. Genet. Genomics 276, 135 - 146.
  38.  Ishikawa,Y., Tanaka, N., Murakami, K., Kugoh, H., Oshimura, M., Calos, M.P., and Sugamura, K. (2006). Genomic integration of the common cytokine receptor gamma chain in human T cell lines mediated by phage C31 integrase. J. Gene Med. 8, 646 - 653.
  39.  Chalberg, T.W., Portlock, J.L., Olivares, E.C., Thyagarajan, B., Kirby, P., Hoelters, J., Hillman, R.T., and Calos, M.P. (2006). Integration specificity of phage C31 integrase in the human genome. J. Mol. Biol. 357, 28-48.
  40.  Bertoni, C., Jarrahian, S., Wheeler, T.M., Li, Y., Olivares, E.C., Calos, M.P., and Rando, T.A. (2006). Enhancement of plasmid-mediated gene therapy for Duchenne muscular dystrophy by directed plasmid integration. Proc. Natl. Acad. Sci. 103, 419-424.
  41.  Ginsburg, D. and Calos, M.P. (2005). Site-specific integration for prolonged expression. In Non-viral Vectors for Gene Therapy, Second Edition, Part II (L. Huang, M. Hung, and E. Wagner, eds.) Adv. Genetics. Vol. 54. 177-185.
  42.  Thyagarajan, B. and Calos, M.P. (2005). Site-specific integration for high-level protein production in mammalian cells. In Methods in Molecular Biology, vol. 308: Therapeutic Proteins: Methods and Protocols, Chapter 9, pp. 99-106 (C M Smales and D C James, eds.), Humana Press, Totowa, NJ.
  43.  Ginsburg, D., Thyagarajan, B., Phillips, J.E., and Calos, M.P. (2005). Gene delivery by viruses. In: Encyclopedia of Life Sciences. London: Nature Publishing Group. http://www.els.net/
  44.  Chalberg, T., Phillips, J.E and Calos, M.P. (2005). Transfection of DNA into mammalian cells in culture. In: Encyclopedia of Life Sciences. London: Nature Publishing Group. http://www.els.net/
  45.  Chalberg, T.W., Genise, H., Vollrath, D., and Calos, M.P. (2005). C31 integrase confers genomic integration and long-term transgene expression in rat retina. Investigative Ophthalmology & Visual Science 46, 2140-2146.
  46.  Held, P.K, Olivares, E.C., Aguilar, C.P., Finegold, M., Calos, M.P., and Grompe M. (2005). In vivo correction of murine hereditary tyrosinemia type I by C31 integrase-mediated gene delivery. Molec. Ther. 11, 399-408.
  47.  Quenneville, S.P., Chapdelaine, P., Rousseau, J., Beaulieu, J., Caron, N., Skuk, D., Mills, P., Olivares, E.C., Calos, M.P., and Tremblay, J.P. (2004). Nucleofection of muscle-derived stem cells and myoblasts with phiC31 integrase: stable expression of a full-length-dystrophin fusion gene by human myoblasts. Molec. Ther. 10, 679-687
  48.  Dorigo, O., Gil, J.S., Gallaher, S.D., Tan, B.T., Castro, M.G., Lowenstein, P.R., Calos, M.P., and Berk, A.J. (2004). Development of a novel helper dependent adenovirus-Epstein-Barr virus hybrid system for the stable transformation of mammalian cells. J. Virol. 78, 6556-6566.
  49.  Groth, A.C., Fish, M., Nusse, R., and Calos, M.P. (2004). Construction of transgenic Drosophila by using the site-specific integrase from phage C31. Genetics 166, 1775-1782.
  50.  Groth, A. C. and Calos, M.P. (2004). Phage integrases: biology and applications. J. Mol. Biol. 335, 667-678.
  51.  Olivares, E.C. and Calos, M.P. (2003). Phage C31 integrase-mediated site-specific integration for gene therapy. Gene Therapy and Regulation 2, 103-120.
  52.  Stoll, S.M., Meuse, L., Kay, M.A., and Calos, M.P. (2003). Role of EBV and genomic sequences in gene expression from extrachromosomal gene therapy vectors in mouse liver. Gene Therapy and Molecular Biology 7, 211-219.
  53.  Hollis, R.P., Stoll, S.M., Sclimenti, C.R., Lin, J., Chen-Tsai, Y., and Calos, M.P. (2003). Phage integrases for construction and manipulation of transgenic mammals. Reprod. Biol. and Endocrinol., 1:79.
  54.  Portlock, J.L. and Calos, M.P. (2003). Site-specific genomic strategies for gene therapy. Curr. Opin. Molec. Therapeutics 5, 376- 382.
  55.  Ortiz-Urda, S., Thyagarajan, B., Keene, D., Lin, Q., Calos, M.P., & Khavari, P. A. (2003). C31 integrase-mediated nonviral genetic correction of junctional epidermolysis bullosa. Human Gene Therapy, 14, 923-928.
  56.  Sclimenti, C.R., Neviaser, A.S., Baba, E.J., Meuse, L., Kay, M.A., and Calos, M.P. (2003). Epstein-Barr virus vectors provide prolonged robust factor IX expression in mice. Biotechnol. Prog. 19, 144-151.
  57.  Olivares, E.C., Hollis, R.P., Chalberg, T.W., Meuse, L., Kay, M.A., and Calos, M.P. (2002). Site-specific genomic integration produces therapeutic factor IX levels in mice. Nature Biotech. 20, 1124-1128.
  58.  Ortiz-Urda, S., Thyagarajan, B., Keene, D.R., Lin, Q., Fang, M., Calos, M.P., & Khavari, P. A. (2002). Stable nonviral genetic correction of inherited human skin disease. Nature Med. 8, 1166-1170.
  59.  Stoll, S.M. and Calos, M.P. (2002). Extrachromosomal plasmid vectors for gene therapy. Curr. Opinion Mol. Therapeutics 4, 299-305.
  60.  Stoll, S.M., Ginsburg, D.S., and Calos M.P. (2002). Phage TP901-1 site-specific integrase functions in human cells. J. Bacteriol. 184, 3657-3663.
  61.   Sclimenti, C.R., Thyagarajan, B., and Calos, M.P. (2001). Directed evolution of a recombinase for improved genomic integration at a native human sequence. Nucleic Acids Res. 29, 5044-5051.
  62.   Olivares, E.C., Hollis, R.P., and Calos, M.P. (2001). Phage R4 integrase mediates site-specific integration in human cells. Gene 278, 167-176.
  63.   Stoll, S., Sclimenti, C.R., Baba, E.J., Meuse, L., Kay, M.A., & Calos, M.P. (2001). Epstein-Barr Virus/ human vector provides high-level long-term expression of 1-antitrypsin in mice. Molecular Therapy 4, 122-129.
  64.   Thyagarajan, B., Olivares, E.C., Hollis, R.P., Ginsburg, D.S., & Calos, M.P. (2001). Site-specific genomic integration in mammalian. cells mediated by phage C31 integrase. Mol. Cell. Biol. 21, 3926-3934.
  65.   Thyagarajan, B., Phillips, J.E., and Calos, M.P. (2001). Gene delivery by viruses. In: Encyclopedia of Life Sciences. London: Nature Publishing Group. http://www.els.net/
  66.   Sclimenti, C.R., Baba, E.J., and Calos, M.P. (2000). An extrachromosomal tetracyline-regulatable system for mammalian cells. Nucleic Acids Res. 28, e80.
  67.   Groth, A.C., Olivares, E.C., Thyagarajan, B., and Calos, M.P. (2000). A phage integrase directs efficient site-specific integration in human cells. Proc. Natl. Acad. Sci. 97, 5995-6000.
  68.   Thyagarajan, B., Guimaraes, J., Groth, A.C. and Calos, M.P. (2000). Mammalian genomes contain active recombinase recognition sites. Gene 244, 47-54.
  69.   Phillips, J.E and Calos, M.P (2000). Transfection of DNA into mammalian cells in culture. In: Encyclopedia of Life Sciences. London: Nature Publishing Group. http://www.els.net/
  70.   Phillips, J.E. and Calos, M.P. (1999). Effects of homology length and donor vector arrangement on the efficiency of double-strand break-mediated recombination in human cells. Somat. Cell and Molec. Genet. 25, 91-100.
  71.   Phillips, J.E., Thyagarajan, B., and Calos, M.P. (1999). Epstein-Barr virus plasmid model system for analyzing recombination in human cells. Plasmid 41, 198-206.
  72.   Sclimenti, C.R. and Calos, M.P. (1998). Epstein-Barr virus vectors for gene expression and transfer. Current Opinion Biotechnology 9, 476-479.
  73.   Calos, M.P. (1998). Stability without a centromere. Proc. Natl. Acad. Sci. 95, 4084-4085.
  74.   Bulboaca, G.H., Deng, H., Dewhurst, S., and Calos, M.P. (1998). Telomeric sequences from human herpesvirus 6 do not mediate nuclear retention of episomal DNA in human cells. Archives of Virol. 143, 563-570.
  75.   Sclimenti, C.R. and Calos, M.P. (1998). Assaying extrachromosomal gene therapy vectors that carry persistence/replication elements. Advanced Drug Delivery Rev. 30, 13-21.
  76.   Calos, M.P. (1996). The potential of extrachromosomal replicating vectors in gene therapy. Trends in Genetics 12, 463-466.
  77.   Wohlgemuth, J.G., Kang, S., Bulboaca, G., Nawotka, K., and Calos, M.P. (1996). Long-term gene expression from autonomously replicating vectors in mammalian cells. Gene Therapy 3, 503-512.
  78.   Smith, J.G., Caddle, M.S., Bulboaca, G.H., Wohlgemuth, J.G., Baum, M., Clarke, L., and Calos, M.P. (1995). Replication of centromere II of Schizosaccaromyces pombe. Mol. Cell. Biol. 14, 5165-5172.
  79.   Smith, J.G. and Calos, M.P. (1995). Autonomous replication in Drosophila melanogaster tissue culture cells. Chromosoma 103, 597-605.
  80.   Wohlgemuth, J.G., Bulboaca, G.H., Moghadam, M., Caddle, M.S., and Calos, M.P. (1994). Physical mapping of origins of replication in Schizosaccaromyces pombe. Mol. Biol. Cell 5, 839-849.
  81.   Haase, S.B., Heinzel, S.S., and Calos, M.P. (1994). Transcription inhibits the replication of autonomously replicating plasmids in human cells. Mol. Cell. Biol. 14, 2516-2524
  82.   Caddle, M.S. and Calos, M.P. (1994). Specific initiation at an origin of replication from Schizosaccharomyces pombe. Mol. Cell. Biol. 14, 1796-1805.
  83.   Krysan, P.J. and Calos, M.P. (1993). Epstein-Barr virus-based vectors that replicate in rodent cells. Gene 136, 137-144.
  84.   Krysan, P.J., Smith, J.G., and Calos, M.P. (1993). Autonomous replication in human cells of multimers of specific human and bacterial DNA sequences. Mol. Cell. Biol., 13, 2688 - 2696.
  85.   Tran, C.T., Caddle, M.S., and Calos, M.P. (1993). The replication behavior of Saccharomyces cerevisiae DNA in human cells. Chromosoma 102, 129-136.
  86.   Caddle, M.S. and Calos, M.P. (1992). Analysis of the autonomous replication behavior in human cells of the dihydrofolate reductase putative chromosomal origin of replication. Nucleic Acids Res. 20,5971-5978.
  87.   Haase, S.B. and Calos, M.P. (1991). Replication control of autonomously replicating human sequences. Nucleic Acids Res. 19, 5053-5058.
  88.   Heinzel, S.S., Krysan, P.J., Tran, C.T., and Calos, M.P. (1991). Autonomous replication in human cells is affected by the size and the source of the DNA. Mol. Cell. Biol. 11, 2263-2272.
  89.   Krysan, P.J. and Calos, M.P. (1991). Replication initiates at multiple locations on an autonomously replicating plasmid in human cells. Mol. Cell. Biol. 11, 1464-1472.
  90.   Calos, M.P. (1990). The molecular biology of mutation in human cells: From shuttle vectors to artificial chromosomes. (Eleventh Bristol-Myers Symp. On Cancer Research), In The Cellular and Molecular Biology of Human Carcinogenesis, Chapter 7, pp.139-151.
  91.   Krysan, P.J., Haase, S.B., and Calos, M.P. (1989). Isolation of human sequences which replicate autonomously in human cells. Mol. Cell. Biol., 9, 1026-1033.
  92.   Hsia, H.C., Lebkowski, J.S., Leong, P.M., Calos, M.P., and Miller, J.H. (1989). Comparison of ultraviolet light mutagenesis of the lacI gene in E. coli and in human 293 cells. J. Mol. Biol. 205, 103-113.
  93.   Haase, S.B., Heinzel, S.S., Krysan, P.J., and Calos, M.P. (1989). Improved EBV shuttle vectors. Mutation Research 220. 125-132.
  94.   Calos, M.P. (1988). Use of lacI shuttle systems for analysis of mutation in human cells. In DNA Repair, Vol. 3, eds. Friedberg, E.C. and Hanawalt, P.C., Marcel Dekker, New York, chapter 13, pp. 277-294.
  95.   Heinzel, S.S., Krysan, P.J., Calos, M.P., and DuBridge, R.B. (1988). Use of simian virus 40 replication to amplify Epstein-Barr virus shuttle vectors in human cells. J. Virol. 62, 3738-3746.
  96.   DuBridge, R.B. and Calos, M.P. (1988). Recombinant shuttle vectors for the study of mutation in human cells. Mutagenesis 3, 1-9.
  97.   Miller, J.H., and Calos, M.P. (1987). Gene Transfer Vectors for Mammalian Cells. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. (Book)
  98.   Van Den Berg, D.J., DuBridge, R.B., and Calos, M.P. (1987). EBV-lacI shuttle vectors: Addition of a selection for LacI mutants. In Banbury Report 28: Mammalian Cell Mutagenesis, pp. 307-314.
  99.   DuBridge, R.B. and Calos, M.P. (1987). Molecular approaches to the study of mutation in human cells. Trends in Genetics, 3, 293-297.
  100.   DuBridge, R.B., Tang, P., Hsia, H.C., Leong, P.-M., Miller, J.H., and Calos, M.P. (1987). Analysis of mutation in human cells using an Epstein-Barr virus shuttle system. Mol. Cell. Biol. 7, 379-387.
  101.   Calos, M.P. (1986). Mutation of autonomously replicating plasmids. In Gene Transfer, ed. R. Kucherlapati, Plenum Press, New York, pp. 243-261.
  102.   Lebkowski, J.S., Miller, J.H., and Calos, M.P. (1986). Determination of DNA sequence changes induced by ethyl methanesulfonate in human cells, using a shuttle vector system. Mol. Cell. Biol. 6, 1838-1842.
  103.   Lebkowski, J.S., Clancy, S., Miller, J.H., and Calos, M.P. (1985). The lacI shuttle: Rapid analysis of the mutagenic specificity of ultraviolet light in human cells. Proc. Natl. Acad. Sci. 82, 8606-8610.
  104.   DuBridge, R.B., Lusky, M., Botchan, M.R., and Calos, M.P. (1985). Amplification of a bovine papilloma virus-simian virus 40 chimera. J. Virol. 56, 625-627.
  105.   Lebkowski, J.S., Clancy, S., and Calos, M.P. (1985). SV40 replication in adenovirus-transformed human cells antagonizes gene expression. Nature 317, 169-171.
  106.   Lebkowski, J.S., Botchan, M.R., and Calos, M.P. (1983). Spontaneous mutation of DNA transfected into mammalian cells. In Cellular Responses to DNA Damage, UCLA Symposia on Molecular and Cellular Biology, New Series, Vol. 11, eds. Friedberg, E.C. and Bridges, B., Alan R. Liss, New York, pp. 603-614.
  107.   Miller, J.H., Lebkowski, J.S., Greisen, K.S., and Calos, M.P. (1984). Specificity of mutations induced in transfected DNA by mammalian cells. EMBO J. 13, 3117-3121.
  108.   Lebkowski, J.S., DuBridge, R.B., Antell, E.A., Greisen, K.S., and Calos, M.P. (1984). Transfected DNA is mutated in monkey, mouse, and human cells. Mol. Cell. Biol. 4, 1951-1960.
  109.   Clancy, S., Mann, C., Davis, R.W., and Calos, M.P. (1984). Deletion of plasmid sequences during Saccharomyces cerevisiae transformation. J. Bact. 159, 1065-1067.
  110.   Calos, M.P. (1983). Book review of Mobile Genetic Elements, ed. J.A. Shapiro, Science 221, 1366-1367.
  111.   Calos, M.P., Lebkowski, J.S., and Botchan, M.R. (1983). High mutation frequency in DNA transfected into mammalian cells. Proc. Natl. Acad. Sci USA 82, 3015-3019.
  112.   Albertini, A.M., Hofer, M., Calos, M.P., Tlsty, T.D., and Miller, J.H. (1983). Analysis of spontaneous deletions and gene amplification in the lac region of E. coli. Cold Spring Harbor Symp. Quant. Biol. 47, 841-850
  113.   Albertini, A., Hofer, M., Calos, M.P., and Miller, J.H. (1982). On the formation of spontaneous deletions: The importance of short sequence homologies in the generation of large deletions. Cell 29, 319-328.
  114.   Miller, J.H., Calos, M.P., Combepine, C., and Hofer, M. (1981). Appendix: Further correlations of the lacI genetic map with the DNA sequence. J. Mol. Biol. 153, 65-66.
  115.   Calos, M.P. and Miller, J.H. (1981). Genetic and sequence analysis of frameshift mutations induced by ICR-191. J. Mol. Biol. 153, 39-64.
  116.   Calos, M.P. and Miller, J.H. (1981). The DNA sequence change resulting from the IQ1 mutation, which greatly increases promoter strength. Mol. Gen. Genet. 183, 559-560.
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Last updated on
November 12, 2012