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Stress sensing; protein localization |
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Bacteria respond to a variety of individual stresses by switching on a common set of genes that makes them resistant to stresses in general. Little is known about how the different stresses are sensed. We isolated a mutant in Pseudomonas putida that lost the capacity to develop general resistance. The mutated was identified to be flhF, which encodes a G-protein. This location of this protein was traced to the flagella-bearing pole of the cell. The nature of the cascade activated by this protein in response to stresses is under investigation.
Pandza. S., M. Baetens, C-H. Park, T. Au, M. Keyhan, and A. Matin.
2000. The putative G-protein FLHF has a role in polar flagellar placement
and general stress response induction in Pseudomonas putida. Molecular
Microbiology 36: 414-42 [PDF] A.
Flagellar location and the location of the FlhF protein as determined by fusing the latter to the green fluorescent protein. Note that in dividing cells, flagella are located at the pole distal to the division septum, i.e., the pole where the fluorescence is seen.
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