Some Schemes for SM Imaging in Cells
FCS
fluorescent ligand
bound to TM
protein
fusions bound to repeated promoters
lipid analogs
fusions to FPs
labeled CPPs
Methods:
wide-field,
TIRF,
confocal
two-photon…
enzymatic conversion
of fluorogens (
b
-lactamase,
b
-galactosidase, …)
turn-on fluorophores
(FlAsH, …)
labeled viruses
molecular
beacons
W. E. Moerner