Single-Molecule Light Sources in Living Cells
So Yeon Kim, Zemer Gitai, Anika Kinkhabwala, Lucy
Shapiro, WEM,
PNAS 103, 10929 (2006)
The challenge: look
inside a cell, letting a single molecule tell us what is going on!
Approach:
use fusion between protein of interest (bacterial actin, MreB) and GFP – see movie
link!
ATP
ADP
Treadmilling
actin analogy
Average SM speed ~ 6.0 ± 0.2 nm/s
Þ1.2 monomers added / second
Superresolution
(30 nm) image of MreB filaments!
MreB
bar 1 mm