Single Particle Femtosecond X-ray Diffractive Imaging

We have demonstrated single pulse diffractive imaging of substrate-free biomolecules with a soft X-ray free electron laser. Megadalton duplex DNA complexes electrosprayed from a sucrose-containing solution were injected into single FEL pulses. This work is the direct prelude to single biomolecule imaging with hard X-ray FELs. This work projects that single-pulse single-particle images will be collected at LCLS and the Euro XFEL without further advancement of the technology we reported. We have since used this technology to analyze live cells, nanoparticles, carbon nanofibers, flame- and powder-generated aerosols at FLASH.

References:

Bogan, M., Starodub, D., Hampton, C. Y., Sierra R. G. “Single Particle Coherent Diffractive Imaging with a Soft X-ray Free Electron Laser: Towards Soot Aerosol Morphology” J. Phys. B: Atom., Mol., Optics, 43, 194013, 2010 -invited article to the "8 years of FLASH" special issue >>link to paper>>

Bogan, M. et al. “Single-shot femtosecond X-ray diffraction from identical ellipsoidal nanoparticles in unknown random orientations, Physical Review Special Topics: Accelerators and Beams, 13, 094701, 2010 >>link to paper>>

Loh, D. et al. “Cryptotomography: the synthesis of 3D intensities from randomly oriented single-shot diffraction patterns”, Physical Review Letters, 104, 225501, 2010

Saldin, D. et al. “Structure of a single particle from scattering by many particles randomly oriented about an axis: towards structure solution without crystallization?”, New Journal of Physics, 12, 035014, 2010

Bogan, M.; et al. “Aerosol imaging with a soft X-ray free electron laser”, Aerosol Science and Technology, 44, i-vi, 2010

Saldin, D. K.;Shneerson, V. L.;Starodub, D.;Spence, J. C. H. "Reconstruction from a single diffraction pattern of azimuthally projected electron density of molecules aligned parallel to a single axis" Acta Crystallographica Section A 66, 32, 2010

Chapman, H. N., et al. "Coherent Imaging at FLASH". Journal of Physics: Conf. Ser. 186:012051.29, 2009

Bogan, M.; Benner, WH; Boutet, S.; Rohner, U.; Frank, M.; Barty, A.; Seibert, M. M.; Maia, F. R. N. C.; Marchesini, S.; Bajt, S.; Woods, B. W.; Riot, V.; Hau-Riege, S. P.; Marklund, E.; Spiller, E.; Svenda, M.; Hajdu, J.; Chapman, H. N., "Single particle X-ray diffractive imaging" Nano Letters, 2008, 8, 310-316

 PULSE Research Nanoscale & Biomolecular Imaging • Publications • Scientific Staff