Real-time Interferometric Synthetic Aperture Microscopy: Inverse Scattering for Optical Coherence Tomography

Dr. Tyler S. Ralston, University of Illinois at Urbana-Champaign

Abstract:

State-of-the-art methods in high-resolution three-dimensional (3-D) optical microscopy require that the focus be scanned through the entire region of interest. It is believed that features outside of the focus are inherently unresolvable. Here, a novel computational image-formation technique called interferometric synthetic aperture microscopy (ISAM) is described and demonstrated. A mathematical model is developed connecting the experimentally acquired signal with the three-dimensional object structure, taking into account the finite beam width, diffraction and defocus effects. Using this model, the analytic solution to the inverse scattering problem for coherence microscopy is derived. Through implementation, resolution produced by conventional coherence microscopy at the focus can be achieved for all planes outside of the focus. Explicitly, the structure of an object is determined using all the data collected from the illuminated volume. Numerical simulations show that scatterers can be resolved outside of the confocal volume. Verification with a tissue phantom and human breast tissue demonstrate spatially invariant resolution. These results improve the high-resolution cross-sectional imaging capabilities for 3-D microscopy. Furthermore, the reconstruction algorithm may be implemented for either cross-sectional images or full 3-D volumes, and because of the modest computational complexity of this technique, ISAM is amenable to real-time imaging.

Bio:

Dr. Tyler S. Ralston was born in Dayton, Ohio, in 1977. He received his Bachelors from the Electrical and Computer Engineering Department at the University of Dayton in 2000. Following his degree, he worked in the medical products sector at Battelle Memorial Institute in Columbus, Ohio, earning a Key Contributor award for production of a medical instrument. He received his Masters and Ph.D. from the Electrical and Computer Engineering Department at the University of Illinois at Urbana-Champaign. During his PhD, he held a fellowship at the Beckman Institute for Advanced Science and Technology. He now is a postdoctoral research associate at the same institution. His research interests include both signal processing and inverse problems for optical biomedical imaging. Dr. Ralston has served on the board for the University of Illinois chapters of the IEEE and the OSA as president, vice president, and treasurer.