Journal Publications
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Categories:
On-chip isotachophoresis (ITP)
On-chip isotachophoresis (ITP)
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Eid, C., Garcia-Schwarz, G., Santiago, J. G., “Isotachophoresis with ionic spacer and two-stage separation for high sensitivity DNA hybridization assay,” Analyst 2013. |
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Bahga, S. S., Santiago, J. G., “Coupling isotachophoresis and capillary electrophoresis: a review and comparison of methods,” Analyst 2013. |
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Bahga, S. S., Han, C. M., Santiago, J. G., “Integration of rapid DNA hybridization and capillary zone electrophoresis using bidirectional isotachophoresis,” Analyst 2013, 138, 87-90. |
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Bahga, S. S., Bercovici, M., Santiago, J. G., “Robust and high-resolution simulations of nonlinear electrokinetic processes in variable cross-section channels,” Electrophoresis 2012, 33, 3036-3051. |
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Marshall, L. A., Wu, L. L., Babikian, S., Bachman, M., Santiago, J. G., “Integrated Printed Circuit Board Device for Cell Lysis and Nucleic Acid Extraction,” Analytical Chemistry 2012, 84, 9640-9645. |
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Bercovici, M., Han, C. M., Liao, J. C., Santiago, J. G., “Rapid hybridization of nucleic acids
using isotachophoresis,” |
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Shkolnikov, V., Bahga, S.S., Santiago, J. G., “Desalination and Hydrogen, Chlorine, and Sodium Hydroxide Production via Electrophoretic Ion Exchange and Precipitation,” Supplementary information available here. |
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Garcia-Schwarz, G., Santiago, J. G., “ Integration of On-Chip Isotachophoresis and Functionalized Hydrogels for Enhanced-Sensitivity Nucleic Acid Detection,” Anal. Chem. 2012. Supplementary information available here. |
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Rogacs, A., Qu, Y., and Santiago, J. G., “Bacterial RNA Extraction and Purification from Whole Human Blood Using Isotachophoresis,” Anal. Chem. 2012. |
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Bahga, S. S., and Santiago, J. G., “Concentration Cascade of Leading Electrolyte Using Bidirectional Isotachophoresis,” Electrophoresis 2012, 33, 1048-1059. |
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Garcia-Schwarz, G., Rogacs, A., Bahga, S. S., and Santiago, J. G., “On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids,” Journal of Visualized Experiments 2012, 61, e3890. Click here to watch video. |
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Marshall, L. A., Han, C. M., and Santiago, J. G., “Extraction of DNA from Malaria-Infected Erythrocytes Using Isotachophoresis,” Analytical Chemistry 2011, 83, 9715-9718. |
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M. Bercovici, G.V. Kaigala, K.E. Mach, C.M. Han, J.C. Liao and J.G. Santiago, “Rapid Detection of Urinary Tract Infections Using Isotachophoresis and Molecular Beacons,” Analytical Chemistry 2011, 83, 4110-4117. |
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S.S. Bahga, R.D. Chambers, and J.G. Santiago, “Coupled Isotachophoretic Preconcentration and Electrophoretic Separation Using Bidirectional Isotachophoresis,” Analytical Chemistry 2011. Supplementary information available here. |
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G. Garcia-Schwarz, M. Bercovici, L.A. Marshall, and J.G. Santiago, “Sample dispersion in isotachophoresis,” Supplementary information available here. |
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A. Persat, and J.G. Santiago, “MicroRNA Profiling by Simultaneous Selective Isotachophoresis and Hybridization with Molecular Beacons,” Analytical Chemistry 2011, 83, 2310-2316. |
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S.S. Bahga, G.V. Kaigala, M. Bercovici and J.G. Santiago, “High-sensitivity detection using isotachophoresis with variable cross-section geometry ” Electrophoresis 2010. Supplementary information available here. |
A. Persat, R.R. Chivukula, J.T. Mendell and J.G. Santiago, “Quantification of global microRNA abundance by selective isotachophoresis ” Analytical Chemistry 2010. Supplementary information available here. |
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G.V. Kaigala, M. Bercovici, M. Behnam, D. Elliott, J.G. Santiago, and C.J. Backhouse, “Miniaturized device for isotachophoresis assays,” Lab on a Chip 2010. 10, 2242-2250. Supplementary information available here. |
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S.S. Bahga, M. Bercovici, and J.G. Santiago, “Ionic strength effects on electrophoretic Supplementary information available here. |
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M. Bercovici, G.V. Kaigala, and J.G. Santiago, “Method for Analyte Identification Using Isotachophoresis and a Fluorescent Carrier Ampholyte Assay,” Analytical Chemistry, 2010. Supplementary information available here. |
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M. Bercovici, G.V. Kaigala, C.J. Backhouse, and J.G. Santiago, “Fluorescent Carrier Ampholytes Assay for Portable, Label-Free Detection of Chemical Toxins in Tap Water,” Analytical Chemistry, 2010. Supplementary information available here. |
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A. Persat, L.A. Marshall, and J.G. Santiago, “Purification of Nucleic Acids from Whole Blood Using Isotachophoresis,” Analytical Chemistry, vol. 81, 2009, pp. 9507-9511. Supplementary information available here. |
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| Persat A., and Santiago J.G., “Electrokinetic control of sample splitting at a channel bifurcation using isotachophoresis,” New Journal of Physics, vol. 11, 2009, p. 075026. | |
| Schoch R.B., Ronaghi M., and Santiago J.G., “Rapid and selective extraction, isolation, preconcentration, and quantitation of small RNAs from cell lysate using on-chip isotachophoresis,” Lab on a Chip, vol. 9, pp. 2145-2152, 2009. | |
| Chambers R. D. and Santiago J.G., "Imaging and Quantification of Isotachophoresis Zones Using Nonfocusing Fluorescent Tracers," Analytical Chemistry, vol. 81, pp. 3022-3028, 2009. | |
| Khurana T.K. and Santiago J.G. , “Effects of carbon dioxide on peak mode isotachophoresis: Simultaneous preconcentration and separation,” Lab on a Chip, vol. 9, 2009, pp. 1377-1384. | |
Bercovici M., Lele S.K., and Santiago J.G. , “Open source simulation tool for electrophoretic stacking, focusing, and separation”, Journal of Chromatography A, 1216, pp. 1008-1018, 2009. Download SPREESSO here. |
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Khurana T., and Santiago J.G., “Sample Zone Dynamics in Peak Mode Isotachophoresis” 80, 16, Analytical Chemistry, pp. 6300-6307, 2008. Supplementary information available here. |
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Khurana T., and Santiago J.G., “Pre-Concentration, Separation, and Indirect Detection of Non-Fluorescent Analytes using Fluorescent Mobility Markers” 80, 1, Analytical Chemistry, pp. 279-286,2008. Supplementary information available here. |
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Jung B., Zhu Y., and Santiago J.G., "Detection of 100 attomolar Fluorophores Using a High Sensitivity On-Chip CE System and Transient Isotachophoresis," Analytical Chemistry, 79, 1, p. 345-349, 2007. Supplementary information available here. |
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Jung, B., Bharadwaj, R., Santiago, J.G., "On-Chip Million-Fold Sample Stacking Using Single-Interface Isotachophoresis," 78, 7, Analytical Chemistry, pp. 2319-2327, 2006. |
Electrophoretic preconcentration techniques (non-ITP)
General work on optimizing on-chip electrokinetic flows and electrophoresis
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D. Milanova, R.D. Chambers, S.S. Bahga and J.G. Santiago, “Effect of PVP on the electroosmotic mobility of wet-etched glass microchannels” Electrophoresis 2012, 33, 3259-3262. |
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D. Milanova, R.D. Chambers, S.S. Bahga and J.G. Santiago, “Electrophoretic mobility measurements of fluorescent dyes using on-chip capillary electrophoresis” Electrophoresis 2011, 32, 3286-3294. |
Persat A., Chambers R.D., and Santiago J.G., “Basic principles of electrolyte chemistry for microfluidic electrokinetics. Part I: Acid-base equilibria and pH buffers,” Lab on a Chip, vol. 9, 2009, pp. 2437-2453. Supplementary information available here. |
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| Persat A., Suss M.E., and Santiago J.G. , “Basic principles of electrolyte chemistry for microfluidic electrokinetics. Part II: Coupling between ion mobility, electrolysis, and acid-base equilibria,” Lab on a Chip, vol. 9, 2009, pp. 2454-2469. | |
Lin, H., B.D. Storey and J.G. Santiago, “A depth-averaged electrokinetic flow model for shallow microchannels,” 608, Journal of Fluid Mechanics, p. 43-70, 2008. |
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Nohmi, M. and JG Santiago, “Quick Measurement of Electroosmotic Flow Velocity,” Lab on a Chip, Chips & Tips, March 2008. |
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Lin, F., F. Baldessari, R. Chambers, C. Crenguta Gyenge, T. Sato, J.G. Santiago, E.C. Butcher, Lymphocyte Electraxis in vitro and in vivo,“ in press, Journal of Immunology, 2007. |
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Persat, A., T. Zangle, J. Posner, and J. Santiago “Chips & Tips: On-chip Electorphoresis Devices: Do’s, Don’ts, and Dooms,” Lab on a Chip, Chips & Tips, March 2007. |
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Santiago, J.G., “Comments on the Conditions for Similitude in Electroosmotic Flows,” 310, 2, p. 675-677, Journal of Colloids and Interface Science, 2007. |
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Matta, A., O.M. Knio, R.G. Ghanem, C.-H. Chen, J.G. Santiago, B. Debusschere, and H.N. Najm, “Computational Study of Band Crossing Reactions,” Journal of Microelectromechanical Systems, 13, 2, pp. 310-322, 2003. |
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Oddy, M. and J.G. Santiago, "Alternating Electric Field Measurements of Particle Zeta-Potentials in a Microchannel," Journal Colloid and Interface Science, 269, pp. 192-204, 2004 |
Mohammadi, B. and J.G. Santiago, “Incomplete Sensitivities in Design and Control of Fluidic Channels,” Computer Assisted Mechanics and Engineering Sciences, 10, pp. 201-210, 2003 |
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Bharadwaj, R., J.G. Santiago, and B. Mohammadi, "Design and Optimization of On-Chip Capillary Electrophoresis," Electrophoresis, 23, pp. 2729-2744, 2002 |
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Mohammadi, B., Molho, J.I., and J.G. Santiago, “Incomplete sensitivities for the design of minimal dispersion fluidic channels,” Computer Method in Applied Mechanics and Engineering, 192, 37-38, pp. 4131-4145, 2002 |
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Mohammadi, B. and J.G. Santiago, “Simulation and Design of Extraction and Separation Fluidic Devices,” Mathematical Modelling and Numerical Analysis, 35, 3, pp. 513-523, 2002 |
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Santiago, J.G., "Electroosmotic Flows in Microchannels with Finite Inertial and Pressure Forces, "Analytical Chemistry, 73, 10, pp. 2353-2365, 2001 |
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Molho, J.I., A.E. Herr, B.P. Mosier, J.G. Santiago, T.W. Kenny, R.A. Brennen, G.B. Gordon, B.Mohammadi, “Optimization of Turn Geometries for On-Chip Electrophoresis,” Analytical Chemistry, 73, 6, pp. 1350-1360, 2001 |
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Herr, A.H., J.I. Molho, J.G. Santiago, M.G. Mungal, T.W. Kenny, and M.G. Garguilo, "Electroosmotic Capillary Flow with Non-Uniform Z-Potential," Analytical Chemistry, 72, 5, pp. 1053-1057, 2000 |
Fuel Cells
Nanochannel Electrokinetic Flows and Assays
| M. E. Suss, T. F. Baumann, W. L. Bourcier, C. M. Spadaccini, K. A. Rose, J. G. Santiago, M. Stadermann, “Capacitive desalination with flow-through electrodes,” Energy and Environmental Science 2012, 5, 9511-9519. | |
| T.A. Zangle, A. Mani, and J.G. Santiago, “Effects of Constant Voltage on Time Evolution of Propagating Concentration Polarization,” Analytical Chemistry 2010, 82, 3114-3117. | |
| F. Baldessari and J.G. Santiago, “Corrigendum to "Electrokinetics in nanochannels. Part I. Electric double layer overlap and channel-to-well equilibrium" [J. Colloid Interface Sci. 325 (2008) 526-538],” Journal of Colloid and Interface Science, vol. 331, Mar. 2009, p. 549. | |
| F. Baldessari and J.G. Santiago, “Corrigendum to "Electrokinetics in nanochannels. Part II. Mobility dependence on ion density and ionic current measurements" [J. Colloid Interface Sci. 325 (2008) 539-546],” Journal of Colloid and Interface Science, vol. 331, Mar. 2009, p. 550. | |
| T.A. Zangle, A. Mani, and J.G. Santiago, “Theory and experiments of concentration polarization and ion focusing at microchannel and nanochannel interfaces,” Chemical Society Reviews, 2010, 39, 1014–1035. | |
| T.A. Zangle, A. Mani, and J.G. Santiago, “On the Propagation of Concentration Polarization from Microchannel−Nanochannel Interfaces Part II: Numerical and Experimental Study,” Langmuir, vol. 25, Mar. 2009, pp. 3909-3916. Supplementary information available here. |
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| A. Mani, T.A. Zangle, and J.G. Santiago, “On the Propagation of Concentration Polarization from Microchannel−Nanochannel Interfaces Part I: Analytical Model and Characteristic Analysis,” Langmuir, vol. 25, Mar. 2009, pp. 3898-3908. | |
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Pennathur, S., F. Baldessari, M. Kattah, J. Steinman, P.J. Utz, and J.G. Santiago, “Free-Solution Oligonucleotide Separation in Nanoscale Channels,“ 79, 21, Analytical Chemistry, p.8316-8322, 2007. |
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Baldessari, F. and J.G. Santiago, "Electrophoresis in nanochannels: brief review and speculation," 4, 12 Nanobiotechnology Journal, doi 10.1186/1477-3155-4-12, 2006. |
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Pennathur, S. and Santiago, J.G. “Electrokinetic transport in nanochannels: 1. Theory,” 77, 21, Analytical Chemistry, pp. 6772-6781, 2005. |
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Pennathur, S. and Santiago, J.G. “Electrokinetic transport in nanochannels: 2. Experiments,” 77, 21, Analytical Chemistry, pp. 6782-6789, 2005. |
Micromixing and Electrokinetic Instabilities
Particle Control, Tracking and Diagnostics
| V.Shkolnikov, J.G. Santiago, "A method for non-invasive full-field imaging and
quantification of chemical species", Lab on a Chip 2013,10.1039
Supplementary information available here. |
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| K.A. Rose, B. Hoffman, D. Saintillan, E.S.G. Shaqfehand J.G. Santiago, "Hydrodynamic interactions in metal rodlike-particle suspensions due to induced charge electroosmosis, Physical Review E 79, 011402, 2009. |
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Piorek, B., S.J. Lee, M. Moskovits, S. Banerjee, J.G. Santiago, and C. Meinhart, “Microfluidic Control of Nanoparticle Aggregation for Surfaced Enhanced Raman Spectroscopy,” 104, 48, Proceedings of the National Academy of Science Journal, p.18898-18901, 2007. |
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Rose, K.A., J.A. Meier, G.M. Dougherty, and J.G. Santiago, “Rotational Electrophoresis of Striped Metallic Microrods,” Physics Review E – Statistical, Nonlinear, and Soft Matter Physics, 75, 1, 2007. |
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Huber, D.E. and Santiago, J.G., “Temperature Gradient Focusing in a Microfluidic Device,” Journal of Heat Transfer, 127, 8, pp. 806, 2005. |
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Devasenathipathy, S., J.G. Santiago, S.T. Wereley, and C.D. Meinhart, "Particle Tracking Techniques for Microfabricated Fluidic Systems," Experiments in Fluids, 34, 4, pp. 504-513, 2003 |
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Mosier, B.P., J.I. Molho, and J.G. Santiago, "Bleached-Fluorescence Imaging of Microflows," Experiments in Fluids, 33, 4, pp. 545-554, 2002 |
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Devasenathipathy, S., J.G. Santiago, and K. Takehara, "Particle Tracking Techniques for Electrokinetic Microchannel Flows," Analytical Chemistry, 74, 15, pp. 3704-3713, 2002 |
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Meinhart, C.D., S.T. Wereley, and J.G. Santiago, "A PIV Algorithm for Estimating Time-Averaged Velocity Fields,” Journal of Fluids Engineering, 122, 2, pp. 285-289, 2000 |
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Meinhart, C.D. , S.T. Wereley, and J.G. Santiago, "PIV Measurements of a Microchannel Flow,"Experiments in Fluids, 27, 5, pp. 414-419, 1999 |
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Santiago, J.G. , S.T. Wereley, C.D. Meinhart, D.J. Beebe, and R.J. Adrian, "A Particle Image Velocimetry System for Microfluidics," Experiments in Fluids, 25, 4, pp. 316-319, 1998 |
Micropumps and Cooling
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Suss, M. E., Mani, A., Zangle, T. A., Santiago, J. G., “Electroosmotic pump performance is affected by concentration polarizations of both electrodes and pump,” Sensors and Actuators A 2011, 165, 310-315. |
S. Litster, M.E. Suss and J.G. Santiago,“A two-liquid electroosmotic pump using low applied voltage and power,” Sensors and Actuators A 2010, 163, 311-314. Supplementary information available here. |
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V. Shkolnikov, D.G. Strickland, D.P. Fenning and J.G. Santiago, “Design and fabrication of porous polymer wick structures, ” Sensors and Actuators B 2010, 150, 556-563. Supplementary information available here. |
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V. Shkolnikov, J. Ramunas and J.G. Santiago, “A self-priming, roller-free, miniature, peristaltic pump operable with a single, reciprocating actuator , ” Sensors and Actuators A 2010, 160, 141-146. Supplementary information available here. |
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| D.G. Strickland, M.E. Suss, T.A. Zangle, and J.G. Santiago, “Evidence shows concentration polarization and its propagation can be key factors determining electroosmotic pump performance,” Sensors and Actuators B: Chemical, 2009. | |
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Kim, D., J.D. Posner, and J.G. Santiago, “High Flow Rate per Power Electroosmotic Pumping using Low Ion Density Solvents,” Sensors and Actuators A: Physical, 141, pp. 201-212, 2008. |
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Lin, C.W., S. Yao, J.D. Posner, A.M. Meyers, and J.G. Santiago, “Toward Orientation Independent Design for as Recombination in Closed-Loop Electroosmotic Pumps,” v.128, no.1, p.334-339, Sensors and Actuators B, 2007. |
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Kenny, T.W., Goodson, K.E., Santiago J.G., Wang, E., Koo, J.-M., Jiang, L., Pop, E., S. Sinha, S. Zhang, L., Fogg, D. et al. “Advanced cooling technologies for microprocessors,” International Journal of High Speed Electronics and Systems, March 2006, 16, 1, p. 301-313. |
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Yao, S., Myers, A.M, Posner, J.D., Rose, K.A., and Santiago, J.G., “Electroosmotic Pumps Fabricated from Porous Silicon Membranes,” 15, 3, Journal of Microelectromechanical Systems, pp. 717-728, 2006. |
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Wang, E.N., Devasenathipathy, S., Lin, H., Hidrovo, C.H., Santiago, J.G., Goodson, K.E., and Kenny T.W., "A Hybrid Method for Bubble Geometry Reconstruction in Two-Phase Microchannels," 40, 6, Experiments in Fluids, p. 847-858, 2006. |
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Tripp, J.A., F. Svec, J.M.J. Frechet, S. Zeng, J.C. Mikkelsen, and J.G. Santiago, “High-Pressure Electroosmotic Pumps Based on Porous Polymer Monoliths,” Sensors and Actuators B, 99, pp. 66-73, 2004 |
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Wang, G.R., J. G. Santiago, M.G. Mungal, B. Young, S. Papademetriou, “A laser-induced cavitation pump," Journal of Micromechanics and Microengineering, 14, 7, pp. 1037-1046, 2004. |
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Wang, E.N., Devasenathipathy, S., Lin, H., Hidrovo, C.H., Santiago, J.G., Goodson, K.E., and Kenny, T.W., "Nucleation and growth of vapor bubbles in a heated silicon microchannel," Journal of Heat Transfer, 126, p. 497, 2004. |
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Laser, D. and J.G. Santiago, “A Review of Micropumps,” Journal of Micromechanics and Microengineering, 14, 6, 2004 |
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Yao, S. and J.G. Santiago , “Porous Glass Electroosmotic Pumps: Theory, ” Journal of Colloid and Interface Science, 268, pp. 133-142, 2003 |
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Yao, S., D.E. Hertzog, S. Zeng, J.C. Mikkelsen, and J.G. Santiago , “Porous Glass Electroosmotic Pumps: Design and Experiments,” Journal of Colloid and Interface Science, 268, pp. 143-153, 2003 |
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Chen, C.-H. and J.G. Santiago, “A Planar Electoosmotic Micropump,” Journal of Microelectromechanical Systems, 11, No.6, pp. 672-683, 2002 |
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Jiang, L., J.C. Mikkelsen, J.-M. Koo, D. Huber, S. Yao, L. Zhang, P. Zhou, Maveety, R. Prasher, J.G. Santiago, T.W. Kenny, and K.E. Goodson, "Closed-Loop Electroosmotic Microchannel Cooling System for VLSI Circuits," IEEE Transactions on Components & Packaging Technologies, 25, 3, pp.347-355, 2002 |
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Zhang, L., J.M. Koo, L. Jiang, M. Ashegi, K.E. Goodson, J.G. Santiago, T.W. Kenny, "Measurements and Modeling of Two-Phase Flow in Microchannels with Nearly Constant Heat Flux Boundary Conditions,” Journal of Microelectromechanical Systems, 11, 1, pp. 12-19, 2002 |
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Zeng, S., J.G. Santiago, J.-R. Chen, R.N. Zare, F. Svec, J.M.J. Frechet, "Electroosmotic Flow Pumps with Polymer Frits," Sensors and Actuators B, 82, Nos. 2-3, pp. 209-212, 2001 |
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Zeng, S., C.-H. Chen, J. C. Mikkelsen, J.G. Santiago, "Fabrication and Characterization of Electroosmotic Micropumps," Sensors and Actuators B, 79, pp. 107-114, 2001 |
