Machine Name
    ui600
    Location
    Stauffer I 17B
    Hardware
    600 MHz NMR spectrometer
    Varian Inova console
    Sun Blade 100
    Software
    Solaris 2.8
    VNMR 6.1C
    Primary Uses of Instrument
    1) NMR of Macromolecules
    2) All 2D and 3D experiments except 1H/ 13C/ 31P experiments
    3) Solvent Suppression 2D experiments
    4) Low sensitivity 2D experiments including natural abundance             1H/15N HSQC/HMBC
    5) 2D experiments on low concentration samples (<~1 nM)
    Probe
    5mm Triple Resonance Pulsed Field Gradient
        HCN
        Ideal for Proton detect experiments, especially 2D and 3D                 experiments
        Including: All protein and RNA pack sequences except                     1H/13C/31P experiments
 


Setting the VT Temperature
Probes for Inova 600
Why Tune the Probe?
How to Tune the Probe
Gradient Shimming
Calibrate 1H 90* Pulse
Calibrate 13C 90* Pulse for Indirect Detect
Types of Two Dimensional Experiments
Set-Up Macros for Two Dimensional Experiments
Parameters that Affect Length of 2D Experiment
HSQC
HMQC
HMBC
NOESY
ROESY
TOCSY
Data Processing
Data Display

 

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