Machine Name
    ui500
    Location
    Stauffer I 18A
    Hardware

   500 MHz NMR spectrometer
   Varian Inova console
   Sun Blade 100

    Software
    Solaris 2.8
    VNMR 6.1C
 
    Primary Uses of Instrument
    1) 1D 1H, 13C, 19F, 31P, other nuclei
    2) 1D NOE
    3) 1D with variable temperature (-100º - 150ºC)
    4) All 2D experiments including COSY, TOCSY, HSQC, HMBC,        ROESY, NOESY
    5) Solvent Suppression
    Probes
    5mm PFG Switchable (Standard Probe)
       X/H
       Tunable to X nuclei from 31P (202 MHz) to 15N (50 MHz)
       1H channel tunable to 19F
       Ideal for detection of broadband nuclei
       Can be used for most 2D experiments including COSY,           TOCSY, HSQC, HMBC, ROESY, NOESY, but less sensitive           than inverse type probes

    5mm Triple Resonance/Triax Pulsed Field Gradient
       HCN
       Ideal for Proton detect experiments, especially 2D and 3D           experiments
       Including:
       NOESY/ROESY/TOCSY (1H/1H)
       HMQC/HSQC/HMBC (1H/13C or 1H/15N)

    3mm Triple Resonance/Z Pulsed Field Gradient
       HCN
       Ideal for Proton detect experiments with small sample volumes

    5mm Double Resonance/Z Pulsed Gradient
       H/X
       Ideal for Proton detect experiments especially 2D and 3D
          experiments, using X nucleus other than 13C or 15N
       Including:
       31P/1H Heteronuclear COSY/TOCSY
 


1D Detection of Nuclei Other than 1H or 13C
What Probe Should You Choose?
Probes for Inova 500
Setting the VT Temperature
Why Tune the Probe?
How to Tune the Probe
Gradient Shimming
Calibrate 1H 90* Pulse
Calibrate 13C 90* Pulse for Indirect Detect
Types of Two Dimensional Experiments
Set-Up Macros for Two Dimensional Experiments
Parameters that Affect Length of 2D Experiment
HSQC
HMQC
HMBC
NOESY
ROESY
TOCSY
Data Processing
Data Display

 

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