Tuning the Probe

First, the software needs to tell the hardware which channel is which, so a pulse sequence must be setup (su) with the proper channels - for an HSQC or HMBC or HMQC:
Transmitter/channel 1- 1H, Decoupler/channel 2- 13C, Decoupler/channel 3- 15N.

For a 1D 13C detect:
Transmitter/channel 1- 13C, Decoupler/channel 2- 1H

Next the cables to the probe must be inserted into the tuning interface/preamp box.

Next plug the output cable of the tuning/preamp box to the tuning output below the tune meter (necessary on ui500 but not ui600 or ui300). Turn on the tuning meter to channel 1, sensitivity scale 9. Adjust the two gold (inner and outer) knobs on the probe that say proton to minimize the number on the digital tune meter. You should be able to tune down to either 000 or 001. If you are having trouble getting below a certain number, deliberately make one of the knobs worse and adjust the other one to minimize, if you cannot get to the number you were previously at, turn that knob back the other way, and minimize the second knob.

After tuning to 001, then turn off tune meter by turning it to channel 0, disconnect/reconnect the cables and return them to their original position. 13C should also be tuned (and 15N if there are 15N pulses). Either 13C or 15N are done similarly except now the broadband side of the tune/preamp box is used, plug the 13C cable from the probe into the tune interface and the cable going to the broadband output hope into the tune output. 13C should be tuned on channel 2 (HSQC), 15N on channel 3; like 1H, they should be tunable to 001 on the sensitivity scale of 9.