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10X MEMFA Salts 1 liter 1M Mops - 100 ml EGTA - 2 ml MgSO4 - 1 ml Note – do not Autoclave
Triethanolamine 3 liters 55.5g powder 3000 ml H20 pH - 7-8
Hybridization Buffer 1 liter (Final Conc.) Formamide - 500 ml (50%) 20X SSC - 250 ml (5X) Torula RNA* -1 g (1mg/ml) Heparin - 100 mg (100µg/ml) Denhart’s - 10 ml (1X) Tween 20 - 1 g (0.1%) CHAPS - 1 g (0.1%) EDTA(0.5M)- 20 ml (10mM) H20 (upto 1 L) Store @ -20 ° C*(add Torula RNA powder at the end)
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10X MAB 1 liter to 500 ml H20 add Maleic Acid - 116g NaCl - 87g NaOH pellets - 55g pH to 7.5
PBT 8 liters 10X PBS - 800 ml Tween 20 - 8 ml H20 - 7192 ml
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PBT 1 liter 100 ml - 10X PBS 899 ml - dH20 1ml - Tween-20
4% Paraformaldehyde 500 ml 400 ml - PBTween 100 ml - 20% Paraformaldehyde
Alkaline phosphate buffer (make fresh) 100 ml Tris 9.5 (1M) - 10 ml MgCl2 (1M) - 5 ml NaCl - 0.6 g Tween-20 - 100 µl
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