Sea Urchin Embryology Back to Contents page

 

 

DETERGENTS

You will need to make a serial dilution of the detergent of your choice [start with a liquid one, it's much easier]

TUBE #

INSTRUCTIONS

DILUTION

1

1 part detergent & 9 parts seawater

1 : 10

2

1 part tube #1 & 9 parts seawater

1 : 100

3

1 part tube #2 & 9 parts seawater

1 : 1,000

4

1 part tube #3 & 9 parts seawater

1 : 10,000

5

1 part tube #4 & 9 parts seawater

1 : 100,000

6

1 part tube #5 & 9 parts seawater

1 : 1,000,000

7

1 part tube #6 & 9 parts seawater

1 : 10,000,000

8

PURE SEAWATER

CONTROL

Set out 8 test tubes with 1/2 inch of each of the above. Then:

  1. Add one drop of concentrated eggs to each.
  2. Swirl and let sit for one minute.
  3. Add one drop of diluted sperm suspension and swirl briefly.
  4. Wait 5 minutes.
  5. Score eggs for fertilization. If time permits can also score for later development [Next period, see if divided. Next day see if there are swimming blastula.]