Detergents
You will need to make a serial dilution of the detergent of your choice [start with a liquid one, it's much easier]
Tube # |
Instructions |
Dilution |
1 |
1 part detergent & 9 parts seawater |
1 : 10 |
2 |
1 part tube #1 & 9 parts seawater |
1 : 100 |
3 |
1 part tube #2 & 9 parts seawater |
1 : 1,000 |
4 |
1 part tube #3 & 9 parts seawater |
1 : 10,000 |
5 |
1 part tube #4 & 9 parts seawater |
1 : 100,000 |
6 |
1 part tube #5 & 9 parts seawater |
1 : 1,000,000 |
7 |
1 part tube #6 & 9 parts seawater |
1 : 10,000,000 |
8 |
PURE SEAWATER |
CONTROL |
Set out 8 test tubes with 1/2 inch of each of the above. Then:
- Add one drop of concentrated eggs to each.
- Swirl and let sit for one minute.
- Add one drop of diluted sperm suspension and swirl briefly.
- Wait 5 minutes.
- Score eggs for fertilization. If time permits can also score for later development [Next period, see if divided. Next day see if there are swimming blastula.]