Copper
Copper compounds are common in industrial situations. They are also used in anti-fouling paints on boats in the ocean.
You will need to make a serial dilution of the copper stock solution [1 gram of copper sulfate in 100 ml of seawater] [so starting at 1:100]
|
TUBE # |
INSTRUCTIONS |
DILUTION |
| 1 |
1 part stock & 9 parts seawater |
1 : 1000 |
| 2 |
1 part tube #1 & 9 parts seawater |
1 : 10,000 |
| 3 |
1 part tube #2 & 9 parts seawater |
1 : 100,000 |
| 4 |
1 part tube #3 & 9 parts seawater |
1 : 1,000,000 |
| 5 |
1 part tube #4 & 9 parts seawater |
1 : 10,000,000 |
| 6 |
1 part tube #5 & 9 parts seawater |
1 : 100,000,000 |
| 7 |
1 part tube #6 & 9 parts seawater |
1 : 1,000,000,000 |
| 8 |
PURE SEAWATER |
CONTROL |
Set out 8 test tubes with 1/2 inch of each of the above. Then:
- Add one drop of concentrated eggs to each.
- Swirl and let sit for one minute.
- Add one drop of diluted sperm suspension and swirl briefly.
- Wait 5 minutes.
- Score eggs for fertilization. If time permits can also score for later development [Next period see if divided. Next day see if there are swimming blastula]